Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Ligation Related Discussions
  1. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  2. Gel for my ligation - (reply: 1)
  3. PCR after T4 ligation? - (reply: 3)
  4. Restriction and ligation time interval - (reply: 1)
  5. Help needed for Ligation/Transformation - (reply: 1)
  6. Ligation failure? I ran some on a gel - (reply: 6)
  7. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
  8. Ligation left for weekend at room temp - (reply: 4)
  9. How to store a ligation reaction over the weekend? - (reply: 3)
  10. HELP - Adaptor ligation to DNA not working! - (reply: 1)
  11. Insert & vector too dilute to perform ligation - (reply: 3)
  12. cloning- ligation - (reply: 3)
  13. Sticky and blunt ligation issue - (reply: 6)
  14. i couldn't get the band of target gene after ligation - (reply: 9)
  15. Problems with ligation and transformation - (reply: 2)
  16. PCR Profile for ligation - (reply: 3)
  17. ligation transformation - (reply: 4)
  18. Ligation problem - (reply: 10)
  19. I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
  20. Ligation of PCR fragments - (reply: 11)
  21. ligation - (reply: 8)
  22. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  23. TOPO TA ligation problems - (reply: 6)
  24. Smallest possible insert size for Ligation reaction - (reply: 2)
  25. Adaptor ligation - (reply: 9)
  26. self ligation - (reply: 1)
  27. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  28. Ligation with small insert and large vector - (reply: 6)
  29. TA cloning: ligation problem or toxic construct? - (reply: 4)
  30. Is ligation really necessary? - (reply: 6)
  31. problem with ligation of linker/adaptor - (reply: 2)
  32. Ligation troubleshooting! Please help! - (reply: 5)
  33. Screening for ligation and transformation result - (reply: 2)
  34. Problem with 3 way ligation - (reply: 2)
  35. ligation with one ristrection site/enzyme - (reply: 3)
  36. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
  37. Help for pGEM-T easy vector ligation problem - (reply: 2)
  38. Ligation: two basic questions - (reply: 1)
  39. Confirmation of ligation - (reply: 7)
  40. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  41. Ligation of large ammount of insert (no transformation) - (reply: 7)
  42. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
  43. Transformation or Ligation problems - (reply: 4)
  44. DNA ligation and trasformation - (reply: 6)
  45. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  46. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  47. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  48. Difficult Ligation - Details Inside - (reply: 9)
  49. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
  50. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  51. Three way ligation problem - (reply: 6)
  52. problems with recovery of dna from gel and ligation - (reply: 5)
  53. Ligation - (reply: 20)
  54. help ,blunt end ligation - (reply: 4)
  55. Subcloning - RE digest and ligation and transformation - (reply: 5)
  56. DNA sequencing after ligation! HELP! - (reply: 8)
  57. T4 Ligation Failing - (reply: 8)
  58. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  59. Clarification on digesting a ligation mixture! - (reply: 1)
  60. Ligation into a BAC? - (reply: 2)
  61. should I do blunt end my pcr product before ligation - (reply: 2)
  62. having trouble getting insert ligation into vector - (reply: 3)
  63. Cloning Problem - Ligation or Transformation - (reply: 4)
  64. Double Linker ligation is it possible? - (reply: 3)
  65. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  66. Problems with blunt end ligation - (reply: 3)
  67. Heat inactivate ligase - (reply: 5)
  68. three part ligation with sticky ends not working! - (reply: 2)
  69. Ligations..im not getting a happy "sticky" ending :( - (reply: 3)
  70. Blunt end ligation insert:vector ratio - (reply: 1)
  71. ligation colonies contain empty vector.. - (reply: 9)
  72. Ligation Problem - (reply: 4)
  73. Ligation gel picture interpretation - (reply: 4)
  74. Ligation of insert to itself problem - (reply: 3)
  75. Help with ligation - (reply: 2)
  76. Blunt end PCR product ligation - (reply: 1)
  77. Issues with ligation - (reply: 2)
  78. Ligation of double insert into pET31 failing - (reply: 1)
  79. Ligation of 16kb PCR product - (reply: 5)
  80. Ligation needed after SDM with PfuX? - (reply: 3)
  81. Ligation problem - (reply: 16)
  82. Problem with Blunt end ligation - (reply: 4)
  83. Selection of clones after ligation? - (reply: 11)
  84. ligation of pcr product into expression vector - (reply: 4)
  85. Lenti vector ligation - (reply: 2)
  86. Three-way ligation in two steps? - (reply: 3)
  87. Ligation problem - (reply: 7)
  88. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  89. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
  90. Loss of promoter during ligation - (reply: 2)
  91. ligation and transformation problem - (reply: 1)
  92. Lambda DNA ligation - (reply: 1)
  93. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  94. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
  95. Problems with ligation and transformation - (reply: 2)
  96. Ligation and transformation problem - (reply: 2)
  97. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  98. Self-circularization-How to prevent other unwanted ligations - (reply: 5)
  99. Ligation - (reply: 2)
  100. SmaI blunt end ligation problem... - (reply: 3)
  101. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
  102. Optimum Molar ratio for 3-way ligation - (reply: 5)
  103. HELP!! 3 way ligation question - (reply: 1)
  104. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  105. Adapter ligation problem - (reply: 5)
  106. how to set up the ligation - (reply: 14)
  107. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  108. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  109. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  110. Amplification of concatenated linear ligated fragments - (reply: 4)
  111. How to prevent concatemerization during ligation reaction? - (reply: 2)
  112. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  113. CIP controls for ligation reaction look really weird - (reply: 2)
  114. Question: Ligation Problem.... - (reply: 2)
  115. Ligation problem, religation of backbone without any insert? - (reply: 3)
  116. problem with pLK01 plasmid ligation - (reply: 2)
  117. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  118. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
  119. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
  120. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  121. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  122. Cloning problems i.e. Ligation - (reply: 2)
  123. cleanup of digestion before ligation - (reply: 6)
  124. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  125. blunt ligation - (reply: 4)
  126. Help! my plasmid size changed after ligation - (reply: 5)
  127. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  128. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  129. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  130. Ligation Problem? Or Something else...? - (reply: 3)
  131. Ligation of Blunt PCR Product - (reply: 1)
  132. ligation problem - (reply: 3)
  133. ligation of an adaptor into a vector - (reply: 4)
  134. digestion of insert twice and ligation of 3 bits - (reply: 6)
  135. blunt end ligation problem - Self ligation and truncate (reply: 1)
  136. neb ligase - (reply: 2)
  137. Yet another ligation problem - (reply: 6)
  138. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  139. Problem in Ligation - (reply: 12)
  140. multiway ligation - (reply: 6)
  141. Blunt end ligation - Cant get ligation to work (reply: 4)
  142. blunt ligation - (reply: 1)
  143. Ligation problem - ligation (reply: 19)
  144. ligation problem - (reply: 6)
  145. Failed Ligation - (reply: 2)
  146. Ligation problem - (reply: 9)
  147. ligation/transformation failed - (reply: 7)
  148. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  149. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  150. Ligation screening - (reply: 4)
  151. Double digestion and ligation problem - (reply: 1)
  152. T4 ligase buffer - (reply: 4)
  153. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  154. ligation problems - no colonies at the end!!!!! (reply: 4)
  155. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  156. Ligation - (reply: 3)
  157. Ligation Problems - (reply: 2)
  158. ligation - (reply: 1)
  159. different copy # multimers with linker ligation? - (reply: 2)
  160. Anybody did any AscI ligation? - (reply: 5)
  161. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  162. ligation problem - (reply: 6)
  163. ligation problem - (reply: 3)
  164. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  165. problem in ligation and cloning - (reply: 1)
  166. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  167. Storage of gel extracted DNA before ligation - (reply: 3)
  168. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  169. Ligation Problems - (reply: 2)
  170. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
  171. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  172. Triple ligation not working - (reply: 1)
  173. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  174. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
  175. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  176. Yet another ligation problem - (reply: 4)
  177. is too much ligation reaction bad for transformation? - (reply: 4)
  178. Ligation Time and Temp - (reply: 4)
  179. PCR and then ligation - (reply: 7)
  180. 3 way ligation help needed - (reply: 10)
  181. DNA ligation - (reply: 2)
  182. ligation for 24 hrs in 4*C? - (reply: 3)
  183. Different ligation ratios - (reply: 1)
  184. Troubles with cloning - No positive ligation (reply: 3)
  185. Cloning by multiple oligo ligation - (reply: 2)
  186. multi way ligation or long range pcr? - (reply: 1)
  187. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  188. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  189. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
  190. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  191. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  192. Ligation trouble continues - subcloning ligation failure (reply: 9)
  193. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  194. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  195. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  196. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  197. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  198. Ligation issue: Does vector size matter? - (reply: 10)
  199. help wih the ligation - (reply: 7)
  200. blunt end ligation problem - (reply: 2)
  201. blunt ligation - (reply: 3)
  202. Problems with my ligation reaction - (reply: 5)
  203. PCR problems on a ligation product - (reply: 5)
  204. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  205. optimum temperature for ligation - (reply: 14)
  206. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  207. Ligation problem (no colonies, but on negative control) - (reply: 3)
  208. no ligation !!!!! - (reply: 6)
  209. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  210. ligation - (reply: 5)
  211. Ligation problem - (reply: 9)
  212. Ligation issues - (reply: 4)
  213. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  214. ligation probs - (reply: 2)
  215. help with ligation/transformation - (reply: 1)
  216. ligation problem with SalI degested vector and insert - (reply: 6)
  217. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  218. ligation problem - (reply: 3)
  219. ligation question - three-point ligation (reply: 9)
  220. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  221. No band in the gel after ligation - (reply: 6)
  222. Troubleshooting Digest, ligation, transformation - (reply: 7)
  223. growth of colonies after ligation - (reply: 2)
  224. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
  225. Ligation after SalI & XhoI Digestion - (reply: 3)
  226. ligation problem - (reply: 11)
  227. ds-oligo cloning/ligation issues - (reply: 18)
  228. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
  229. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  230. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
  231. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
  232. gel purification or ligation problem - (reply: 3)
  233. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
  234. Ligation very large vector and small insert - It will not work... (reply: 7)