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Ligation Related Discussions
  1. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  2. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  3. Sticky and blunt end ligation in single reaction? - (reply: 2)
  4. Ligation and blue white screening - (reply: 1)
  5. No colonies after self Circularization ligation - (reply: 2)
  6. Clean ligation controls but no insert - (reply: 12)
  7. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  8. Gel for my ligation - (reply: 1)
  9. PCR after T4 ligation? - (reply: 3)
  10. Restriction and ligation time interval - (reply: 1)
  11. Help needed for Ligation/Transformation - (reply: 1)
  12. Ligation failure? I ran some on a gel - (reply: 6)
  13. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
  14. Ligation left for weekend at room temp - (reply: 4)
  15. How to store a ligation reaction over the weekend? - (reply: 3)
  16. HELP - Adaptor ligation to DNA not working! - (reply: 1)
  17. Insert & vector too dilute to perform ligation - (reply: 3)
  18. cloning- ligation - (reply: 3)
  19. Sticky and blunt ligation issue - (reply: 6)
  20. i couldn't get the band of target gene after ligation - (reply: 9)
  21. Problems with ligation and transformation - (reply: 2)
  22. PCR Profile for ligation - (reply: 3)
  23. ligation transformation - (reply: 4)
  24. Ligation problem - (reply: 10)
  25. I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
  26. Ligation of PCR fragments - (reply: 11)
  27. ligation - (reply: 8)
  28. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  29. TOPO TA ligation problems - (reply: 6)
  30. Smallest possible insert size for Ligation reaction - (reply: 2)
  31. Adaptor ligation - (reply: 9)
  32. self ligation - (reply: 1)
  33. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  34. Ligation with small insert and large vector - (reply: 6)
  35. TA cloning: ligation problem or toxic construct? - (reply: 4)
  36. Is ligation really necessary? - (reply: 6)
  37. problem with ligation of linker/adaptor - (reply: 2)
  38. Ligation troubleshooting! Please help! - (reply: 5)
  39. Screening for ligation and transformation result - (reply: 2)
  40. Problem with 3 way ligation - (reply: 2)
  41. ligation with one ristrection site/enzyme - (reply: 3)
  42. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
  43. Help for pGEM-T easy vector ligation problem - (reply: 2)
  44. Ligation: two basic questions - (reply: 1)
  45. Confirmation of ligation - (reply: 7)
  46. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  47. Ligation of large ammount of insert (no transformation) - (reply: 7)
  48. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
  49. Transformation or Ligation problems - (reply: 4)
  50. DNA ligation and trasformation - (reply: 6)
  51. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  52. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  53. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  54. Difficult Ligation - Details Inside - (reply: 9)
  55. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
  56. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  57. Three way ligation problem - (reply: 6)
  58. problems with recovery of dna from gel and ligation - (reply: 5)
  59. Ligation - (reply: 20)
  60. help ,blunt end ligation - (reply: 4)
  61. Subcloning - RE digest and ligation and transformation - (reply: 5)
  62. DNA sequencing after ligation! HELP! - (reply: 8)
  63. T4 Ligation Failing - (reply: 8)
  64. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  65. Clarification on digesting a ligation mixture! - (reply: 1)
  66. Ligation into a BAC? - (reply: 2)
  67. should I do blunt end my pcr product before ligation - (reply: 2)
  68. having trouble getting insert ligation into vector - (reply: 3)
  69. Cloning Problem - Ligation or Transformation - (reply: 4)
  70. Double Linker ligation is it possible? - (reply: 3)
  71. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  72. Problems with blunt end ligation - (reply: 3)
  73. Heat inactivate ligase - (reply: 5)
  74. three part ligation with sticky ends not working! - (reply: 2)
  75. Ligations..im not getting a happy "sticky" ending :( - (reply: 3)
  76. Blunt end ligation insert:vector ratio - (reply: 1)
  77. ligation colonies contain empty vector.. - (reply: 9)
  78. Ligation Problem - (reply: 4)
  79. Ligation gel picture interpretation - (reply: 4)
  80. Ligation of insert to itself problem - (reply: 3)
  81. Help with ligation - (reply: 2)
  82. Blunt end PCR product ligation - (reply: 1)
  83. Issues with ligation - (reply: 2)
  84. Ligation of double insert into pET31 failing - (reply: 1)
  85. Ligation of 16kb PCR product - (reply: 5)
  86. Ligation needed after SDM with PfuX? - (reply: 3)
  87. Ligation problem - (reply: 16)
  88. Problem with Blunt end ligation - (reply: 4)
  89. Selection of clones after ligation? - (reply: 11)
  90. ligation of pcr product into expression vector - (reply: 4)
  91. Lenti vector ligation - (reply: 2)
  92. Three-way ligation in two steps? - (reply: 3)
  93. Ligation problem - (reply: 7)
  94. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  95. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
  96. Loss of promoter during ligation - (reply: 2)
  97. ligation and transformation problem - (reply: 1)
  98. Lambda DNA ligation - (reply: 1)
  99. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  100. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
  101. Problems with ligation and transformation - (reply: 2)
  102. Ligation and transformation problem - (reply: 2)
  103. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  104. Self-circularization-How to prevent other unwanted ligations - (reply: 5)
  105. Ligation - (reply: 2)
  106. SmaI blunt end ligation problem... - (reply: 3)
  107. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
  108. Optimum Molar ratio for 3-way ligation - (reply: 5)
  109. HELP!! 3 way ligation question - (reply: 1)
  110. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  111. Adapter ligation problem - (reply: 5)
  112. how to set up the ligation - (reply: 14)
  113. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  114. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  115. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  116. Amplification of concatenated linear ligated fragments - (reply: 4)
  117. How to prevent concatemerization during ligation reaction? - (reply: 2)
  118. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  119. CIP controls for ligation reaction look really weird - (reply: 2)
  120. Question: Ligation Problem.... - (reply: 2)
  121. Ligation problem, religation of backbone without any insert? - (reply: 3)
  122. problem with pLK01 plasmid ligation - (reply: 2)
  123. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  124. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
  125. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
  126. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  127. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  128. Cloning problems i.e. Ligation - (reply: 2)
  129. cleanup of digestion before ligation - (reply: 6)
  130. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  131. blunt ligation - (reply: 4)
  132. Help! my plasmid size changed after ligation - (reply: 5)
  133. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  134. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  135. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  136. Ligation Problem? Or Something else...? - (reply: 3)
  137. Ligation of Blunt PCR Product - (reply: 1)
  138. ligation problem - (reply: 3)
  139. ligation of an adaptor into a vector - (reply: 4)
  140. digestion of insert twice and ligation of 3 bits - (reply: 6)
  141. blunt end ligation problem - Self ligation and truncate (reply: 1)
  142. neb ligase - (reply: 2)
  143. Yet another ligation problem - (reply: 6)
  144. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  145. Problem in Ligation - (reply: 12)
  146. multiway ligation - (reply: 6)
  147. Blunt end ligation - Cant get ligation to work (reply: 4)
  148. blunt ligation - (reply: 1)
  149. Ligation problem - ligation (reply: 19)
  150. ligation problem - (reply: 6)
  151. Failed Ligation - (reply: 2)
  152. Ligation problem - (reply: 9)
  153. ligation/transformation failed - (reply: 7)
  154. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  155. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  156. Ligation screening - (reply: 4)
  157. Double digestion and ligation problem - (reply: 1)
  158. T4 ligase buffer - (reply: 4)
  159. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  160. ligation problems - no colonies at the end!!!!! (reply: 4)
  161. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  162. Ligation - (reply: 3)
  163. Ligation Problems - (reply: 2)
  164. ligation - (reply: 1)
  165. different copy # multimers with linker ligation? - (reply: 2)
  166. Anybody did any AscI ligation? - (reply: 5)
  167. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  168. ligation problem - (reply: 6)
  169. ligation problem - (reply: 3)
  170. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  171. problem in ligation and cloning - (reply: 1)
  172. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  173. Storage of gel extracted DNA before ligation - (reply: 3)
  174. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  175. Ligation Problems - (reply: 2)
  176. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
  177. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  178. Triple ligation not working - (reply: 1)
  179. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  180. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
  181. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  182. Yet another ligation problem - (reply: 4)
  183. is too much ligation reaction bad for transformation? - (reply: 4)
  184. Ligation Time and Temp - (reply: 4)
  185. PCR and then ligation - (reply: 7)
  186. 3 way ligation help needed - (reply: 10)
  187. DNA ligation - (reply: 2)
  188. ligation for 24 hrs in 4*C? - (reply: 3)
  189. Different ligation ratios - (reply: 1)
  190. Troubles with cloning - No positive ligation (reply: 3)
  191. Cloning by multiple oligo ligation - (reply: 2)
  192. multi way ligation or long range pcr? - (reply: 1)
  193. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  194. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  195. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
  196. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  197. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  198. Ligation trouble continues - subcloning ligation failure (reply: 9)
  199. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  200. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  201. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  202. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  203. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  204. Ligation issue: Does vector size matter? - (reply: 10)
  205. help wih the ligation - (reply: 7)
  206. blunt end ligation problem - (reply: 2)
  207. blunt ligation - (reply: 3)
  208. Problems with my ligation reaction - (reply: 5)
  209. PCR problems on a ligation product - (reply: 5)
  210. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  211. optimum temperature for ligation - (reply: 14)
  212. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  213. Ligation problem (no colonies, but on negative control) - (reply: 3)
  214. no ligation !!!!! - (reply: 6)
  215. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  216. ligation - (reply: 5)
  217. Ligation problem - (reply: 9)
  218. Ligation issues - (reply: 4)
  219. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  220. ligation probs - (reply: 2)
  221. help with ligation/transformation - (reply: 1)
  222. ligation problem with SalI degested vector and insert - (reply: 6)
  223. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  224. ligation problem - (reply: 3)
  225. ligation question - three-point ligation (reply: 9)
  226. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  227. No band in the gel after ligation - (reply: 6)
  228. Troubleshooting Digest, ligation, transformation - (reply: 7)
  229. growth of colonies after ligation - (reply: 2)
  230. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
  231. Ligation after SalI & XhoI Digestion - (reply: 3)
  232. ligation problem - (reply: 11)
  233. ds-oligo cloning/ligation issues - (reply: 18)
  234. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
  235. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  236. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
  237. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
  238. gel purification or ligation problem - (reply: 3)
  239. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
  240. Ligation very large vector and small insert - It will not work... (reply: 7)