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Ligation Related Discussions
  1. Can someone help me with my test question regarding ligation mixture? - (reply: 5)
  2. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  3. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  4. Sticky and blunt end ligation in single reaction? - (reply: 2)
  5. Ligation and blue white screening - (reply: 1)
  6. No colonies after self Circularization ligation - (reply: 2)
  7. Clean ligation controls but no insert - (reply: 13)
  8. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  9. Gel for my ligation - (reply: 1)
  10. PCR after T4 ligation? - (reply: 3)
  11. Restriction and ligation time interval - (reply: 1)
  12. Help needed for Ligation/Transformation - (reply: 1)
  13. Ligation failure? I ran some on a gel - (reply: 6)
  14. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
  15. Ligation left for weekend at room temp - (reply: 4)
  16. How to store a ligation reaction over the weekend? - (reply: 3)
  17. HELP - Adaptor ligation to DNA not working! - (reply: 1)
  18. Insert & vector too dilute to perform ligation - (reply: 3)
  19. cloning- ligation - (reply: 3)
  20. Sticky and blunt ligation issue - (reply: 6)
  21. i couldn't get the band of target gene after ligation - (reply: 9)
  22. Problems with ligation and transformation - (reply: 2)
  23. PCR Profile for ligation - (reply: 3)
  24. ligation transformation - (reply: 4)
  25. Ligation problem - (reply: 10)
  26. I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (reply: 1)
  27. Ligation of PCR fragments - (reply: 11)
  28. ligation - (reply: 8)
  29. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  30. TOPO TA ligation problems - (reply: 6)
  31. Smallest possible insert size for Ligation reaction - (reply: 2)
  32. Adaptor ligation - (reply: 9)
  33. self ligation - (reply: 1)
  34. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  35. Ligation with small insert and large vector - (reply: 6)
  36. TA cloning: ligation problem or toxic construct? - (reply: 4)
  37. Is ligation really necessary? - (reply: 6)
  38. problem with ligation of linker/adaptor - (reply: 2)
  39. Ligation troubleshooting! Please help! - (reply: 5)
  40. Screening for ligation and transformation result - (reply: 2)
  41. Problem with 3 way ligation - (reply: 2)
  42. ligation with one ristrection site/enzyme - (reply: 3)
  43. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
  44. Help for pGEM-T easy vector ligation problem - (reply: 2)
  45. Ligation: two basic questions - (reply: 1)
  46. Confirmation of ligation - (reply: 7)
  47. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  48. Ligation of large ammount of insert (no transformation) - (reply: 7)
  49. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  50. Transformation or Ligation problems - (reply: 4)
  51. DNA ligation and trasformation - (reply: 6)
  52. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  53. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  54. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  55. Difficult Ligation - Details Inside - (reply: 9)
  56. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
  57. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  58. Three way ligation problem - (reply: 6)
  59. problems with recovery of dna from gel and ligation - (reply: 5)
  60. Ligation - (reply: 20)
  61. help ,blunt end ligation - (reply: 4)
  62. Subcloning - RE digest and ligation and transformation - (reply: 5)
  63. DNA sequencing after ligation! HELP! - (reply: 8)
  64. T4 Ligation Failing - (reply: 8)
  65. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  66. Clarification on digesting a ligation mixture! - (reply: 1)
  67. Ligation into a BAC? - (reply: 2)
  68. should I do blunt end my pcr product before ligation - (reply: 2)
  69. having trouble getting insert ligation into vector - (reply: 3)
  70. Cloning Problem - Ligation or Transformation - (reply: 4)
  71. Double Linker ligation is it possible? - (reply: 3)
  72. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  73. Problems with blunt end ligation - (reply: 3)
  74. Heat inactivate ligase - (reply: 5)
  75. three part ligation with sticky ends not working! - (reply: 2)
  76. Ligations..im not getting a happy "sticky" ending :( - (reply: 3)
  77. Blunt end ligation insert:vector ratio - (reply: 1)
  78. ligation colonies contain empty vector.. - (reply: 9)
  79. Ligation Problem - (reply: 4)
  80. Ligation gel picture interpretation - (reply: 4)
  81. Ligation of insert to itself problem - (reply: 3)
  82. Help with ligation - (reply: 2)
  83. Blunt end PCR product ligation - (reply: 1)
  84. Issues with ligation - (reply: 2)
  85. Ligation of double insert into pET31 failing - (reply: 1)
  86. Ligation of 16kb PCR product - (reply: 5)
  87. Ligation needed after SDM with PfuX? - (reply: 3)
  88. Ligation problem - (reply: 16)
  89. Problem with Blunt end ligation - (reply: 4)
  90. Selection of clones after ligation? - (reply: 11)
  91. ligation of pcr product into expression vector - (reply: 4)
  92. Lenti vector ligation - (reply: 2)
  93. Three-way ligation in two steps? - (reply: 3)
  94. Ligation problem - (reply: 7)
  95. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  96. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
  97. Loss of promoter during ligation - (reply: 2)
  98. ligation and transformation problem - (reply: 1)
  99. Lambda DNA ligation - (reply: 1)
  100. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  101. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
  102. Problems with ligation and transformation - (reply: 2)
  103. Ligation and transformation problem - (reply: 2)
  104. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  105. Self-circularization-How to prevent other unwanted ligations - (reply: 5)
  106. Ligation - (reply: 2)
  107. SmaI blunt end ligation problem... - (reply: 3)
  108. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
  109. Optimum Molar ratio for 3-way ligation - (reply: 5)
  110. HELP!! 3 way ligation question - (reply: 1)
  111. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  112. Adapter ligation problem - (reply: 5)
  113. how to set up the ligation - (reply: 14)
  114. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  115. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  116. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  117. Amplification of concatenated linear ligated fragments - (reply: 4)
  118. How to prevent concatemerization during ligation reaction? - (reply: 2)
  119. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  120. CIP controls for ligation reaction look really weird - (reply: 2)
  121. Question: Ligation Problem.... - (reply: 2)
  122. Ligation problem, religation of backbone without any insert? - (reply: 3)
  123. problem with pLK01 plasmid ligation - (reply: 2)
  124. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  125. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
  126. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
  127. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  128. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  129. Cloning problems i.e. Ligation - (reply: 2)
  130. cleanup of digestion before ligation - (reply: 6)
  131. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  132. blunt ligation - (reply: 4)
  133. Help! my plasmid size changed after ligation - (reply: 5)
  134. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  135. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  136. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  137. Ligation Problem? Or Something else...? - (reply: 3)
  138. Ligation of Blunt PCR Product - (reply: 1)
  139. ligation problem - (reply: 3)
  140. ligation of an adaptor into a vector - (reply: 4)
  141. digestion of insert twice and ligation of 3 bits - (reply: 6)
  142. blunt end ligation problem - Self ligation and truncate (reply: 1)
  143. neb ligase - (reply: 2)
  144. Yet another ligation problem - (reply: 6)
  145. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  146. Problem in Ligation - (reply: 12)
  147. multiway ligation - (reply: 6)
  148. Blunt end ligation - Cant get ligation to work (reply: 4)
  149. blunt ligation - (reply: 1)
  150. Ligation problem - ligation (reply: 19)
  151. ligation problem - (reply: 6)
  152. Failed Ligation - (reply: 2)
  153. Ligation problem - (reply: 9)
  154. ligation/transformation failed - (reply: 7)
  155. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  156. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  157. Ligation screening - (reply: 4)
  158. Double digestion and ligation problem - (reply: 1)
  159. T4 ligase buffer - (reply: 4)
  160. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  161. ligation problems - no colonies at the end!!!!! (reply: 4)
  162. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  163. Ligation - (reply: 3)
  164. Ligation Problems - (reply: 2)
  165. ligation - (reply: 1)
  166. different copy # multimers with linker ligation? - (reply: 2)
  167. Anybody did any AscI ligation? - (reply: 5)
  168. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  169. ligation problem - (reply: 6)
  170. ligation problem - (reply: 3)
  171. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  172. problem in ligation and cloning - (reply: 1)
  173. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  174. Storage of gel extracted DNA before ligation - (reply: 3)
  175. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  176. Ligation Problems - (reply: 2)
  177. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
  178. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  179. Triple ligation not working - (reply: 1)
  180. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  181. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
  182. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  183. Yet another ligation problem - (reply: 4)
  184. is too much ligation reaction bad for transformation? - (reply: 4)
  185. Ligation Time and Temp - (reply: 4)
  186. PCR and then ligation - (reply: 7)
  187. 3 way ligation help needed - (reply: 10)
  188. DNA ligation - (reply: 2)
  189. ligation for 24 hrs in 4*C? - (reply: 3)
  190. Different ligation ratios - (reply: 1)
  191. Troubles with cloning - No positive ligation (reply: 3)
  192. Cloning by multiple oligo ligation - (reply: 2)
  193. multi way ligation or long range pcr? - (reply: 1)
  194. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  195. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  196. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
  197. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  198. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  199. Ligation trouble continues - subcloning ligation failure (reply: 9)
  200. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  201. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  202. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  203. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  204. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  205. Ligation issue: Does vector size matter? - (reply: 10)
  206. help wih the ligation - (reply: 7)
  207. blunt end ligation problem - (reply: 2)
  208. blunt ligation - (reply: 3)
  209. Problems with my ligation reaction - (reply: 5)
  210. PCR problems on a ligation product - (reply: 5)
  211. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  212. optimum temperature for ligation - (reply: 14)
  213. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  214. Ligation problem (no colonies, but on negative control) - (reply: 3)
  215. no ligation !!!!! - (reply: 6)
  216. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  217. ligation - (reply: 5)
  218. Ligation problem - (reply: 9)
  219. Ligation issues - (reply: 4)
  220. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  221. ligation probs - (reply: 2)
  222. help with ligation/transformation - (reply: 1)
  223. ligation problem with SalI degested vector and insert - (reply: 6)
  224. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  225. ligation problem - (reply: 3)
  226. ligation question - three-point ligation (reply: 9)
  227. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  228. No band in the gel after ligation - (reply: 6)
  229. Troubleshooting Digest, ligation, transformation - (reply: 7)
  230. growth of colonies after ligation - (reply: 2)
  231. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
  232. Ligation after SalI & XhoI Digestion - (reply: 3)
  233. ligation problem - (reply: 11)
  234. ds-oligo cloning/ligation issues - (reply: 18)
  235. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
  236. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  237. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
  238. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
  239. gel purification or ligation problem - (reply: 3)
  240. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
  241. Ligation very large vector and small insert - It will not work... (reply: 7)