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Ligation Related Discussions
  1. Double Linker ligation is it possible? - (reply: 1)
  2. Problems with blunt end ligation - (reply: 3)
  3. Heat inactivate ligase - (reply: 5)
  4. three part ligation with sticky ends not working! - (reply: 2)
  5. Ligations..im not getting a happy "sticky" ending :( - (reply: 3)
  6. Blunt end ligation insert:vector ratio - (reply: 1)
  7. ligation colonies contain empty vector.. - (reply: 9)
  8. Ligation Problem - (reply: 4)
  9. Ligation gel picture interpretation - (reply: 4)
  10. Ligation of insert to itself problem - (reply: 3)
  11. Help with ligation - (reply: 2)
  12. Blunt end PCR product ligation - (reply: 1)
  13. Issues with ligation - (reply: 2)
  14. Ligation of double insert into pET31 failing - (reply: 1)
  15. Ligation of 16kb PCR product - (reply: 5)
  16. Ligation needed after SDM with PfuX? - (reply: 3)
  17. Ligation problem - (reply: 16)
  18. Problem with Blunt end ligation - (reply: 4)
  19. Selection of clones after ligation? - (reply: 11)
  20. ligation of pcr product into expression vector - (reply: 4)
  21. Lenti vector ligation - (reply: 2)
  22. Three-way ligation in two steps? - (reply: 3)
  23. Ligation problem - (reply: 7)
  24. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  25. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
  26. Loss of promoter during ligation - (reply: 2)
  27. ligation and transformation problem - (reply: 1)
  28. Lambda DNA ligation - (reply: 1)
  29. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  30. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
  31. Problems with ligation and transformation - (reply: 2)
  32. Ligation and transformation problem - (reply: 2)
  33. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  34. Self-circularization-How to prevent other unwanted ligations - (reply: 5)
  35. Ligation - (reply: 2)
  36. SmaI blunt end ligation problem... - (reply: 3)
  37. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
  38. Optimum Molar ratio for 3-way ligation - (reply: 5)
  39. HELP!! 3 way ligation question - (reply: 1)
  40. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  41. Adapter ligation problem - (reply: 5)
  42. how to set up the ligation - (reply: 14)
  43. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  44. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  45. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  46. Amplification of concatenated linear ligated fragments - (reply: 4)
  47. How to prevent concatemerization during ligation reaction? - (reply: 2)
  48. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  49. CIP controls for ligation reaction look really weird - (reply: 2)
  50. Question: Ligation Problem.... - (reply: 2)
  51. Ligation problem, religation of backbone without any insert? - (reply: 3)
  52. problem with pLK01 plasmid ligation - (reply: 2)
  53. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  54. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
  55. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
  56. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  57. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  58. Cloning problems i.e. Ligation - (reply: 2)
  59. cleanup of digestion before ligation - (reply: 6)
  60. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  61. blunt ligation - (reply: 4)
  62. Help! my plasmid size changed after ligation - (reply: 5)
  63. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  64. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  65. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  66. Ligation Problem? Or Something else...? - (reply: 3)
  67. Ligation of Blunt PCR Product - (reply: 1)
  68. ligation problem - (reply: 3)
  69. ligation of an adaptor into a vector - (reply: 4)
  70. digestion of insert twice and ligation of 3 bits - (reply: 6)
  71. blunt end ligation problem - Self ligation and truncate (reply: 1)
  72. neb ligase - (reply: 2)
  73. Yet another ligation problem - (reply: 6)
  74. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  75. Problem in Ligation - (reply: 12)
  76. multiway ligation - (reply: 6)
  77. Blunt end ligation - Cant get ligation to work (reply: 4)
  78. blunt ligation - (reply: 1)
  79. Ligation problem - ligation (reply: 19)
  80. ligation problem - (reply: 6)
  81. Failed Ligation - (reply: 2)
  82. Ligation problem - (reply: 9)
  83. ligation/transformation failed - (reply: 7)
  84. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  85. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  86. Ligation screening - (reply: 4)
  87. Double digestion and ligation problem - (reply: 1)
  88. T4 ligase buffer - (reply: 4)
  89. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  90. ligation problems - no colonies at the end!!!!! (reply: 4)
  91. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  92. Ligation - (reply: 3)
  93. Ligation Problems - (reply: 2)
  94. ligation - (reply: 1)
  95. different copy # multimers with linker ligation? - (reply: 2)
  96. Anybody did any AscI ligation? - (reply: 5)
  97. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  98. ligation problem - (reply: 6)
  99. ligation problem - (reply: 3)
  100. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  101. problem in ligation and cloning - (reply: 1)
  102. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  103. Storage of gel extracted DNA before ligation - (reply: 3)
  104. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  105. Ligation Problems - (reply: 2)
  106. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
  107. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  108. Triple ligation not working - (reply: 1)
  109. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  110. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
  111. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  112. Yet another ligation problem - (reply: 4)
  113. is too much ligation reaction bad for transformation? - (reply: 4)
  114. Ligation Time and Temp - (reply: 4)
  115. PCR and then ligation - (reply: 7)
  116. 3 way ligation help needed - (reply: 10)
  117. DNA ligation - (reply: 2)
  118. ligation for 24 hrs in 4*C? - (reply: 3)
  119. Different ligation ratios - (reply: 1)
  120. Troubles with cloning - No positive ligation (reply: 3)
  121. Cloning by multiple oligo ligation - (reply: 2)
  122. multi way ligation or long range pcr? - (reply: 1)
  123. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  124. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  125. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
  126. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
  127. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  128. Ligation trouble continues - subcloning ligation failure (reply: 9)
  129. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  130. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  131. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  132. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  133. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  134. Ligation issue: Does vector size matter? - (reply: 10)
  135. help wih the ligation - (reply: 7)
  136. blunt end ligation problem - (reply: 2)
  137. blunt ligation - (reply: 3)
  138. Problems with my ligation reaction - (reply: 5)
  139. PCR problems on a ligation product - (reply: 5)
  140. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  141. optimum temperature for ligation - (reply: 14)
  142. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  143. Ligation problem (no colonies, but on negative control) - (reply: 3)
  144. no ligation !!!!! - (reply: 6)
  145. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  146. ligation - (reply: 5)
  147. Ligation problem - (reply: 9)
  148. Ligation issues - (reply: 4)
  149. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  150. ligation probs - (reply: 2)
  151. help with ligation/transformation - (reply: 1)
  152. ligation problem with SalI degested vector and insert - (reply: 6)
  153. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  154. ligation problem - (reply: 3)
  155. ligation question - three-point ligation (reply: 9)
  156. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  157. No band in the gel after ligation - (reply: 6)
  158. Troubleshooting Digest, ligation, transformation - (reply: 7)
  159. growth of colonies after ligation - (reply: 2)
  160. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
  161. Ligation after SalI & XhoI Digestion - (reply: 3)
  162. ligation problem - (reply: 11)
  163. ds-oligo cloning/ligation issues - (reply: 18)
  164. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
  165. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  166. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
  167. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
  168. gel purification or ligation problem - (reply: 3)
  169. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
  170. Ligation very large vector and small insert - It will not work... (reply: 7)