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Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
1. Splitting cells without centrifugation - (reply: 4)
2. Black dots contaminant in stable cell lines - antibiotics - (reply: 1)
3. cell viability assay for caco-2 and pbmc - (reply: 1)
4. 3T3 L1 cell culture - (reply: 1)
5. Proliferation Inhibitors? - (reply: 1)
6. What microscope magnification to use to count fibroblasts and lymphoblasts - (reply: 1)
7. Immortalizing MEFs with SV40 - (reply: 4)
8. Transfer of (live) tissue - (reply: 2)
9. apoptosis detection - (reply: 1)
10. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
11. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
12. CHO-K1 media - (reply: 1)
13. Importance of certified pyrogen-free tubes? - (reply: 2)
14. Cant seem to detect HA-tag using Immunofluorescence - (reply: 1)
15. Transfection with lipofectamine - (reply: 1)
16. Wound healing assay - (reply: 1)
17. Are Bio-rad gene-pulser cuvettes compatible with a Lonza nucleofector 2b system? - (reply: 1)
18. Recovering cells from ancient sample - (reply: 3)
19. Why are my Huh 7s dying after transfections? - (reply: 1)
20. immunofluorescence staining protocol question - (reply: 1)
21. Importance of reagent expiration dates - (reply: 1)
22. Cell Line confluence level - (reply: 1)
23. too much cell density can influence adhesion to culture plate - (reply: 1)
24. Shipment conditions for cultured cells - (reply: 3)
25. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
26. 3T3-L1 cell contamination - (reply: 3)
27. Secondary goat antirabbit and antimouse conjuagated to alexa showing fluorescenc - (reply: 3)
28. Solubilizing agarose in soft agar assay for absorbance reading - (reply: 1)
29. immunostaining of 3D tissue followed by dissociation - (reply: 1)
30. Culture Tubes for CHO cells - (reply: 1)