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1. Cells adhering to walls of conical tube? - (reply: 4)
2. Trouble with differentiating L6 myoblasts - (reply: 1)
3. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
4. Autoclaving water to go into the incubator - (reply: 5)
5. Contamination in HTC116 culture. - (reply: 1)
6. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
7. How to culture HK-2 cells - (reply: 6)
8. SH-SY5Y and Poly-L-Lysine - (reply: 7)
9. Housekeeper for HEK cells? - (reply: 1)
10. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
11. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
12. calculation on inhibitor treatment - (reply: 1)
13. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
14. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
15. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
16. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
17. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
18. Freezing Cells For Use In Assays - (reply: 2)
19. INS-1E cells migration assay - (reply: 1)
20. Oil red o - IF - (reply: 2)
21. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
22. Primary cell line - (reply: 4)
23. MCF-7 Clumping - (reply: 3)
24. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
25. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
26. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
27. Serial dilution of cells - (reply: 3)
28. Determining the amount of plasmid transfected - (reply: 1)
29. Addition of antibiotic to the culture medium - (reply: 1)
30. HS-5 and HS27A cells - (reply: 1)
2. Trouble with differentiating L6 myoblasts - (reply: 1)
3. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
4. Autoclaving water to go into the incubator - (reply: 5)
5. Contamination in HTC116 culture. - (reply: 1)
6. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
7. How to culture HK-2 cells - (reply: 6)
8. SH-SY5Y and Poly-L-Lysine - (reply: 7)
9. Housekeeper for HEK cells? - (reply: 1)
10. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
11. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
12. calculation on inhibitor treatment - (reply: 1)
13. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
14. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
15. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
16. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
17. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
18. Freezing Cells For Use In Assays - (reply: 2)
19. INS-1E cells migration assay - (reply: 1)
20. Oil red o - IF - (reply: 2)
21. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
22. Primary cell line - (reply: 4)
23. MCF-7 Clumping - (reply: 3)
24. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
25. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
26. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
27. Serial dilution of cells - (reply: 3)
28. Determining the amount of plasmid transfected - (reply: 1)
29. Addition of antibiotic to the culture medium - (reply: 1)
30. HS-5 and HS27A cells - (reply: 1)