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Renal necrosis in mice on a high fat diet? - (Aug/16/2018 )

So I've been working on a research where we had a mice strain C57BL/6J fed on a high lipid died (western diet) And I've come across some strange findings which I wasn't able to find in other papers. I'm thinking it could be necrosis due to hyperlipidemia and the cytokine formations. 

Picture 1: Light microscopy: Notice those dark circle shaped inclusions in the tubules:

https://i.imgur.com/Jgh0a2C.png

Picture 2: Here's how they look up close using TEM microscopy. 

https://i.imgur.com/kF5C6kg.png

If anyone could shed some light on what those could be, I'd be forever thankful. 

-Nina00-

Stained with what in the first image?

 

How was the sample processed?

 

They look like lipid bodies in the cells to me, but I'm no pathologist.

-bob1-

We used toluidine blue to stain it.

We used a regular protocol to fixate it and then put it into an epoxy resin, from where it was cut. 

Thinking about it now, they could be: lysomal lamellar bodies of phospholipidosis (found something on that link underneath)

https://www.researchgate.net/figure/A-Lipid-filled-laminated-Transmission-electron-micograph-shows-profile-of-lysomal_fig3_40686283

-Nina00-