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Top : New Forum Archives (2009-): : Cell-Biology
751. How much sonication power needed? - protein extraction from cells (reply: 2)
752. Paraformaldehyde fixation! - in IHC (reply: 1)
753. 90% confluency - (reply: 12)
754. CM-H2DCFDA for Microscopy - (reply: 5)
755. Cytospin - (reply: 1)
756. Transient expression vector - (reply: 11)
757. problem with Mono Mac1 differentiation - (reply: 3)
758. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
759. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
760. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
761. Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
762. Problem with stable overexpression cell lines - (reply: 3)
763. protein complex and siRNA - (reply: 2)
764. RAW264.7 cells+nitric oxide determination - (reply: 13)
765. opti-mem as a serum starvation media - (reply: 1)
766. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
767. THP-1-Blue-CD14 cells - (reply: 4)
768. MCF-7's not responding to estrogen - (reply: 5)
769. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
770. ADMSC or fibroblast? - (reply: 1)
771. how will i be able to culture tumor cells? - (reply: 3)
772. Anyone familiar with macrophage stimulation? - (reply: 2)
773. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
774. alamar blue - (reply: 4)
775. Luciferase assay - (reply: 3)
776. problems with MTT assay - (reply: 1)
777. lentivirus tranduction of monocytes - (reply: 2)
778. Transfection problem - milky media - (reply: 1)
779. Scratch/Wound Healing Assay Substrate? - (reply: 10)
780. stable transfection problems - stable knockdown (reply: 3)

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