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1. Can serum free medium inactivate trypsin? - (reply: 1)
2. How does the first ribosomal protein get synthesized? - (reply: 2)
3. problem with COV318 ovarian cancer cell line - (reply: 3)
4. thawing effect or passage number? Colony formation varies.. - (reply: 4)
5. timecourse experiment for Western and qPCR - (reply: 2)
6. atom replacement in DNA - (reply: 1)
7. SAOS.2 Cell detachment without using Trypsin. - (reply: 1)
8. H2DCFDA for MDCK cells - (reply: 2)
9. lentivirus production issues - (reply: 1)
10. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
11. Effect of FBS on inhibition of signalling pathways - (reply: 1)
12. Temperature Variation on Cell Growth Experiment - (reply: 1)
13. Cytokines feeding cell culture - (reply: 1)
14. cell confluency in IF - (reply: 2)
15. clonogenic assay for shRNA transfected cells - (reply: 3)
16. No proteins after HeLa cell lysis - (reply: 3)
17. Number of cell - (reply: 5)
18. help with 293T - (reply: 2)
19. RNA contamination in DNA samples - (reply: 2)
20. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
21. Blocking neutrophil binding - (reply: 1)
22. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
23. Question regarding soft agar assay. - (reply: 2)
24. penicillin/streptomycin in cell culture - (reply: 3)
25. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
26. B cells cultures. Immunoglobuline production - (reply: 1)
27. Mitochondrial swelling assay (PTP opening) - (reply: 1)
28. Cell death detection using adenosin kinase - (reply: 5)
29. Transfecting three plasmids into hES cells - (reply: 2)
30. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
2. How does the first ribosomal protein get synthesized? - (reply: 2)
3. problem with COV318 ovarian cancer cell line - (reply: 3)
4. thawing effect or passage number? Colony formation varies.. - (reply: 4)
5. timecourse experiment for Western and qPCR - (reply: 2)
6. atom replacement in DNA - (reply: 1)
7. SAOS.2 Cell detachment without using Trypsin. - (reply: 1)
8. H2DCFDA for MDCK cells - (reply: 2)
9. lentivirus production issues - (reply: 1)
10. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
11. Effect of FBS on inhibition of signalling pathways - (reply: 1)
12. Temperature Variation on Cell Growth Experiment - (reply: 1)
13. Cytokines feeding cell culture - (reply: 1)
14. cell confluency in IF - (reply: 2)
15. clonogenic assay for shRNA transfected cells - (reply: 3)
16. No proteins after HeLa cell lysis - (reply: 3)
17. Number of cell - (reply: 5)
18. help with 293T - (reply: 2)
19. RNA contamination in DNA samples - (reply: 2)
20. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
21. Blocking neutrophil binding - (reply: 1)
22. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
23. Question regarding soft agar assay. - (reply: 2)
24. penicillin/streptomycin in cell culture - (reply: 3)
25. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
26. B cells cultures. Immunoglobuline production - (reply: 1)
27. Mitochondrial swelling assay (PTP opening) - (reply: 1)
28. Cell death detection using adenosin kinase - (reply: 5)
29. Transfecting three plasmids into hES cells - (reply: 2)
30. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)