90% confluency - (Aug/05/2009 )
Hello,
We decided to go till 90% conflency level of human skin fibroblast cells. and theres a problem, the pic one of my lab members showed me for 90% conflency, other one argued that it is 60-70%. My cells are getting ready for harvesting, Experts, would you be able to show me any pic of cells at 90% confluency.
so that i can do it properly,
Thanks,
hahaha, very nice. I completely agree with you - everyone has a different idea of what looks like 60% or 70% etc. I guess it depends largely if you are a glass half full or glass half empty kind of person. Sorry I don't have any pics but interested to see any further posts with pics - may start an interesting discussion.
I don't have any pic of subconfluent cells neither. post a picture of your cells and we will tell you what is the confluence.
(I guess you will have various responses from 70 to 90 !)
Here is the pic i have been shown for 90% confluency level of human skin fibroblasts
epigenetics on Aug 6 2009, 01:17 PM said:
those cells appear closer to 50 than 90%. When fibroblasts approach 90% you will see them in contact with one another and swirls usualy start to form. The plate will be almost entirely covered with only a few empty areas.
Alfred Nobel on Aug 6 2009, 01:52 PM said:
epigenetics on Aug 6 2009, 01:17 PM said:
those cells appear closer to 50 than 90%. When fibroblasts approach 90% you will see them in contact with one another and swirls usualy start to form. The plate will be almost entirely covered with only a few empty areas.
The pic is not coming clean, though my pic what i scanned is very clean. However, If you consider the cytoplasmic area inside, they might not be 50%, but more than that. Whats the percentage? i am not sure.
The problem with confluency is that it is different for different cell lines. I occasionally work with brain cell derived cells that have very long processes and are contact inhibited, so while only 30-40% of the apparent flask area is covered, the cells stop growing having reached 100% confluency by the processes touching.
In terms of area, your cells will be about 50-70%, though the picture is a little unclear and without knowing the cell line it is hard to judge how confluent they actually are.
I agree with those above that the picture you posted looks like between 50-70% confluent.
Add one more vote for the 50-70% confluent!
I would say its more like 60% to me.