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361. STR cell line identification - looking for cell line identification method (reply: 1)
362. Passaging HEK293 cells - adding antibiotics (reply: 1)
363. NP-40 Nuclear Lysis Problem - (reply: 2)
364. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
365. help with ly294002 results - (reply: 2)
366. why no plastic dish for confocal? - (reply: 2)
367. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
368. inverted microscope - (reply: 1)
369. TE buffer in PEI transfection - (reply: 1)
370. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
371. THP maturation and chemotaxis - THP maturation and chemotaxis to lipids. (reply: 3)
372. Virus with serum - viral transfection (reply: 1)
373. Autoclave before adding MgCl2 and MgSO4? - SOB medium receipt (reply: 4)
374. antibody dilution? - (reply: 1)
375. help with cell dilution - (reply: 2)
376. Filter for viral transfection - filter 0.22 or 0.45 (reply: 4)
377. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
378. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
379. HTC116 cells detaching !!!! - (reply: 2)
380. Revival of kasumi-1 cells - (reply: 3)
381. TRIPZ lentiviral production - problems with viral production (reply: 5)
382. Wanted: non-toxic, non-fluorescent viability assay - (reply: 1)
383. weird & curious problem with CHO cell culture - (reply: 3)
384. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
385. culturing caco 2 cells - problems interacted with during the culturing (reply: 1)
386. Gelatin coating for HUVEC - how long? (reply: 1)
387. electroporation, 2-mm or 4-mm? - (reply: 2)
388. a question about PI3K inhibitor - (reply: 1)
389. Premature cell lysis - (reply: 2)
390. difficulties in trypsonizing U937 derived macrophages - (reply: 1)
362. Passaging HEK293 cells - adding antibiotics (reply: 1)
363. NP-40 Nuclear Lysis Problem - (reply: 2)
364. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
365. help with ly294002 results - (reply: 2)
366. why no plastic dish for confocal? - (reply: 2)
367. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
368. inverted microscope - (reply: 1)
369. TE buffer in PEI transfection - (reply: 1)
370. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
371. THP maturation and chemotaxis - THP maturation and chemotaxis to lipids. (reply: 3)
372. Virus with serum - viral transfection (reply: 1)
373. Autoclave before adding MgCl2 and MgSO4? - SOB medium receipt (reply: 4)
374. antibody dilution? - (reply: 1)
375. help with cell dilution - (reply: 2)
376. Filter for viral transfection - filter 0.22 or 0.45 (reply: 4)
377. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
378. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
379. HTC116 cells detaching !!!! - (reply: 2)
380. Revival of kasumi-1 cells - (reply: 3)
381. TRIPZ lentiviral production - problems with viral production (reply: 5)
382. Wanted: non-toxic, non-fluorescent viability assay - (reply: 1)
383. weird & curious problem with CHO cell culture - (reply: 3)
384. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
385. culturing caco 2 cells - problems interacted with during the culturing (reply: 1)
386. Gelatin coating for HUVEC - how long? (reply: 1)
387. electroporation, 2-mm or 4-mm? - (reply: 2)
388. a question about PI3K inhibitor - (reply: 1)
389. Premature cell lysis - (reply: 2)
390. difficulties in trypsonizing U937 derived macrophages - (reply: 1)