Protocol Online logo
Top : New Forum Archives (2009-): : Cell-Biology
781. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
782. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
783. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
784. Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
785. Problem with stable overexpression cell lines - (reply: 3)
786. protein complex and siRNA - (reply: 2)
787. RAW264.7 cells+nitric oxide determination - (reply: 13)
788. opti-mem as a serum starvation media - (reply: 1)
789. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 3)
790. THP-1-Blue-CD14 cells - (reply: 4)
791. MCF-7's not responding to estrogen - (reply: 5)
792. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
793. ADMSC or fibroblast? - (reply: 1)
794. how will i be able to culture tumor cells? - (reply: 3)
795. Anyone familiar with macrophage stimulation? - (reply: 2)
796. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
797. alamar blue - (reply: 4)
798. Luciferase assay - (reply: 3)
799. problems with MTT assay - (reply: 1)
800. lentivirus tranduction of monocytes - (reply: 2)
801. Transfection problem - milky media - (reply: 1)
802. Scratch/Wound Healing Assay Substrate? - (reply: 10)
803. stable transfection problems - stable knockdown (reply: 3)
804. Sterilize DNA preps - (reply: 5)
805. HELP...... for NO (nitric oxide) production - (reply: 5)
806. LNCaP xenografts - need a better protocol (reply: 3)
807. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
808. adeno virus infection - (reply: 5)
809. Lentivirus expression system - get no or low titer of virons (reply: 10)
810. B-cell isolation - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32