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Top : New Forum Archives (2009-): : Cell-Biology
781. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
782. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
783. Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
784. Problem with stable overexpression cell lines - (reply: 3)
785. protein complex and siRNA - (reply: 2)
786. RAW264.7 cells+nitric oxide determination - (reply: 13)
787. opti-mem as a serum starvation media - (reply: 1)
788. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
789. THP-1-Blue-CD14 cells - (reply: 4)
790. MCF-7's not responding to estrogen - (reply: 5)
791. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
792. ADMSC or fibroblast? - (reply: 1)
793. how will i be able to culture tumor cells? - (reply: 3)
794. Anyone familiar with macrophage stimulation? - (reply: 2)
795. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
796. alamar blue - (reply: 4)
797. Luciferase assay - (reply: 3)
798. problems with MTT assay - (reply: 1)
799. lentivirus tranduction of monocytes - (reply: 2)
800. Transfection problem - milky media - (reply: 1)
801. Scratch/Wound Healing Assay Substrate? - (reply: 10)
802. stable transfection problems - stable knockdown (reply: 3)
803. Sterilize DNA preps - (reply: 5)
804. HELP...... for NO (nitric oxide) production - (reply: 5)
805. LNCaP xenografts - need a better protocol (reply: 3)
806. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
807. adeno virus infection - (reply: 5)
808. Lentivirus expression system - get no or low titer of virons (reply: 10)
809. B-cell isolation - (reply: 2)
810. Is this contamination or otherwise? - (reply: 11)

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