Immunocytochemistry - (Sep/08/2009 )
Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before adding the antibody and keep the cells after fixation with 4% PFA?
Postdoc_G on Sep 8 2009, 02:53 PM said:
Yes, I keep them in 1x PBS at 4°C until ready for staining.
Don't keep them for too long though, a week or two maximum.
And don't let them dry out!!!!