Removal of LPS - (Mar/17/2006 )
Hi
I have a protein sample that are contaminatet with LPS I plan on remoweing it on an ionexchange colum. Is that wise.
Best regards Prisme
-prisme-
Methods how you can remove endotoxins are:
Heat sterilization
Treatment of strong acid or alkali
Adsorption on activated carbon, asbestos or barium sulphate
Distillation
Reverse osmosis
Ultrafiltration
Two-phase extraction
Adsorption technique
- Ion-exchange chromatography
- Affinity chromatography (e.g. EndoTrap)
- Size-exclusion chromatography
- Reverse-phase chromatography
-EndoTrap-