PBMC stimulation - (Apr/11/2007 )
Hallo everybody,
I plan to stimulate PBMC with concanavalin A and LPS. However, protocols from internet differ in both recommended time and concentrations used. Does anyone of you have an personal experience with it and can share?
Thanks a lot.
Paja
-Paja-
Hi there!
Depends on what cells you're using (mouse or human - human, I presume), and what you want to look at... I used 1µg/ml LPS for mouse cells and 50ng/ml for human cells, and stimulated them for 2 to 36 hrs. But beware that I used this as a positive control solely, and it was of no importance to me if these were physiological conditions.....
Mike
-jadefalcon-
QUOTE (jadefalcon @ Apr 17 2007, 08:56 PM)
Hi there!
Depends on what cells you're using (mouse or human - human, I presume), and what you want to look at... I used 1µg/ml LPS for mouse cells and 50ng/ml for human cells, and stimulated them for 2 to 36 hrs. But beware that I used this as a positive control solely, and it was of no importance to me if these were physiological conditions.....
Mike
Depends on what cells you're using (mouse or human - human, I presume), and what you want to look at... I used 1µg/ml LPS for mouse cells and 50ng/ml for human cells, and stimulated them for 2 to 36 hrs. But beware that I used this as a positive control solely, and it was of no importance to me if these were physiological conditions.....
Mike
we use 50 ug/ml PHA, and stimulate for 3-5 days
-aniu-