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anyone had used triple layer flasks? questions! - (Jul/11/2007 )

I want to use these triple flasks to make large scale. eg. I use 100medium in a traditional T-175 Flasks for suspension K562 cells.
How much medium I could use for a 175 triple flask?

And basically how much space you should leave in the triple flask to let O2 and Co2 get in?

-cathy-

hi dear
well i had used nunc triple layer flasks.however company recommends much differently, as it depends on conc of cells too. we used 25 ml per layer. just try to look into catalogue of the comapany`s flask u are using, u will get idea from there.
regards
satyam

QUOTE (cathy @ Jul 11 2007, 06:11 PM)
I want to use these triple flasks to make large scale. eg. I use 100medium in a traditional T-175 Flasks for suspension K562 cells.
How much medium I could use for a 175 triple flask?

And basically how much space you should leave in the triple flask to let O2 and Co2 get in?

-satyamka-

QUOTE (satyamka @ Jul 11 2007, 10:47 PM)
hi dear
well i had used nunc triple layer flasks.however company recommends much differently, as it depends on conc of cells too. we used 25 ml per layer. just try to look into catalogue of the comapany`s flask u are using, u will get idea from there.
regards
satyam

QUOTE (cathy @ Jul 11 2007, 06:11 PM)
I want to use these triple flasks to make large scale. eg. I use 100medium in a traditional T-175 Flasks for suspension K562 cells.
How much medium I could use for a 175 triple flask?

And basically how much space you should leave in the triple flask to let O2 and Co2 get in?



oh, my god! we bought NUNC triple layer flasks too. I thought it could take much more wacko.gif ph34r.gif if only take 75ml, that does not make sense for me

-cathy-

we use a total of ~300ml in a triple-tier - operating on the idea that you have 3X the surface area of a singe tier, for which we use 100ml

-aimikins-