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Cryopreservation Related Discussions
  1. Thawing cells - (reply: 2)
  2. Glutaraldehyde solution - ok to freeze thaw or should aliquot? - (reply: 1)
  3. Freezing yeast cells - (reply: 4)
  4. Effects of Freezing Point by Organic Buffers - (reply: 5)
  5. RNA Freeze/Thaw and NanoDrop Concentration - (reply: 1)
  6. rna thawing - (reply: 4)
  7. freezing competent cells without dry ice or liquid N - (reply: 3)
  8. thawing effect or passage number? Colony formation varies.. - (reply: 4)
  9. Freezing viable cells in 96-well format? - (reply: 3)
  10. Freezing Cells For Use In Assays - (reply: 2)
  11. Fixed frozen mouse brain sections crack after thawing, repeatedly - (reply: 3)
  12. Freezing DMSO - (reply: 1)
  13. Cryopreservation of MSC at lower cell density - (reply: 2)
  14. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
  15. suitable DNA extraction and sample freezing for MSP - (reply: 1)
  16. Which is a better way to culture cells after thawing ? - (reply: 2)
  17. Freeze Thawing Chemicals - (reply: 9)
  18. Mouse hearts in OCT - freeze/thaw: damage? - (reply: 1)
  19. Accidental wrong concentration of DMSO for freezing - (reply: 1)
  20. Can i still use freezing medium if i used PES filter to filter my DMSO? - (reply: 1)
  21. Freezing cell lysates for westerns - (reply: 2)
  22. Freezing down brain homogenate a good idea? - (reply: 1)
  23. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  24. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
  25. Dewars for Freezing long term? - (reply: 1)
  26. Freezing bacteria - (reply: 4)
  27. cells die 4-6 days after thawing - (reply: 8)
  28. Freezing cells in DMSO straight into minus 80? - (reply: 4)
  29. Cryopreservation of keratinocytes without a -80oC freezer - (reply: 5)
  30. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
  31. Freezing of Protozoa (Toxoplasma) - (reply: 7)
  32. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  33. Thawing PBMCs - (reply: 3)
  34. Freezing culture media - (reply: 4)
  35. freezing adherant cells - (reply: 2)
  36. freezing adherant cells - (reply: 2)
  37. Freezing proteins at -80 - (reply: 1)
  38. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
  39. Freezing density for NIH 3T3 cells - (reply: 3)
  40. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  41. best freezing method for electrocompetent cells - (reply: 9)
  42. qPCR amplification interference fixed with freezing - (reply: 6)
  43. Freezing cell pellets and minimizing lysis - (reply: 2)
  44. freezing antibody lyophilised - (reply: 2)
  45. freezing samples for microarray - (reply: 1)
  46. Difficulty of thawing H9c2 - (reply: 3)
  47. Cryopreservation of MDCK - (reply: 1)
  48. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  49. Problem with Thawing (Freezing) - (reply: 3)
  50. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  51. Extensive MEF death upon thawing - (reply: 1)
  52. Freezing cells - How long can I keep cells at -80C? (reply: 6)
  53. Thaw HEK293 - (reply: 2)
  54. Accidental thawing of frozen cells - (reply: 3)
  55. Low adherence in thawed HUVEC - HUVEC won't attach after thawing (reply: 4)
  56. Cryopreservation of tissue in 50mL centrifuge tube (Urgent) - (reply: 2)
  57. Freezing PCR product??? - (reply: 1)
  58. Freezing Solution - (reply: 1)
  59. Freezing eluted His tag protein - (reply: 5)
  60. To thaw or not to thaw - (reply: 4)
  61. How often do you thaw new cells? - passage number or time? (reply: 7)
  62. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  63. cell cryopreservation after siRNA transfection - (reply: 1)
  64. Storage of Freezing Media - (reply: 2)
  65. Freezing cells- Media or FBS ? - (reply: 2)
  66. problem on removing coverslip for FISH after liquid N2 freezing - when it's still frozen or after thawing? (reply: 1)
  67. Passage/freezing T-cell line calculation problems - (reply: 3)
  68. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
  69. freezing during RNA isolation step - (reply: 1)
  70. Freezing U937 - (reply: 1)
  71. Freezing THP-1 - (reply: 11)
  72. Freezing primers+SYBR green - (reply: 2)
  73. Thawing and Plating - (reply: 4)
  74. Thawing and Plating - (reply: 1)
  75. please help with Freezing primary cells - (reply: 1)
  76. freezing suspended cells - (reply: 7)
  77. Newbie question: Stability of protein lystates - Freeze/thaw cycles affect phosphate groups? (reply: 2)
  78. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  79. Repeat thawing and freezing of antibiotics? - (reply: 9)
  80. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
  81. Freezing cells, quick question - (reply: 1)
  82. freezing after reducing and boiling - (reply: 10)
  83. Do you lose RNA by quick freezing? - (reply: 5)
  84. freezing of E.coli culture prior DNA prep - (reply: 5)
  85. HACAT cells - very low cell viability after thawing cells (reply: 18)
  86. method of thawing microsomes - (reply: 1)
  87. Freeze thawing RNA - (reply: 4)
  88. freezing of digitonin solutions - (reply: 2)
  89. Tips and FAQs about freezing and thawing cells - Abcam (reply: 26)
  90. Effect of freezing on DNA methylation? - (reply: 6)