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Cryopreservation Related Discussions
  1. is -40 freezer enough for cell freezing? - (reply: 1)
  2. Freezing PBMCs that had been thawed and cultured - (reply: 1)
  3. CO2 and N flushing of bakery products - effect of freezing/defrosting - (reply: 2)
  4. Thawing cells - (reply: 2)
  5. Glutaraldehyde solution - ok to freeze thaw or should aliquot? - (reply: 1)
  6. Freezing yeast cells - (reply: 4)
  7. Effects of Freezing Point by Organic Buffers - (reply: 5)
  8. RNA Freeze/Thaw and NanoDrop Concentration - (reply: 1)
  9. rna thawing - (reply: 4)
  10. freezing competent cells without dry ice or liquid N - (reply: 3)
  11. thawing effect or passage number? Colony formation varies.. - (reply: 4)
  12. Freezing viable cells in 96-well format? - (reply: 3)
  13. Freezing Cells For Use In Assays - (reply: 2)
  14. Fixed frozen mouse brain sections crack after thawing, repeatedly - (reply: 3)
  15. Freezing DMSO - (reply: 1)
  16. Cryopreservation of MSC at lower cell density - (reply: 2)
  17. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
  18. suitable DNA extraction and sample freezing for MSP - (reply: 1)
  19. Which is a better way to culture cells after thawing ? - (reply: 2)
  20. Freeze Thawing Chemicals - (reply: 9)
  21. Mouse hearts in OCT - freeze/thaw: damage? - (reply: 1)
  22. Accidental wrong concentration of DMSO for freezing - (reply: 1)
  23. Can i still use freezing medium if i used PES filter to filter my DMSO? - (reply: 1)
  24. Freezing cell lysates for westerns - (reply: 2)
  25. Freezing down brain homogenate a good idea? - (reply: 1)
  26. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  27. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
  28. Dewars for Freezing long term? - (reply: 1)
  29. Freezing bacteria - (reply: 4)
  30. cells die 4-6 days after thawing - (reply: 8)
  31. Freezing cells in DMSO straight into minus 80? - (reply: 4)
  32. Cryopreservation of keratinocytes without a -80oC freezer - (reply: 5)
  33. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
  34. Freezing of Protozoa (Toxoplasma) - (reply: 7)
  35. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  36. Thawing PBMCs - (reply: 3)
  37. Freezing culture media - (reply: 4)
  38. freezing adherant cells - (reply: 2)
  39. freezing adherant cells - (reply: 2)
  40. Freezing proteins at -80 - (reply: 1)
  41. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
  42. Freezing density for NIH 3T3 cells - (reply: 3)
  43. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  44. best freezing method for electrocompetent cells - (reply: 9)
  45. qPCR amplification interference fixed with freezing - (reply: 6)
  46. Freezing cell pellets and minimizing lysis - (reply: 2)
  47. freezing antibody lyophilised - (reply: 2)
  48. freezing samples for microarray - (reply: 1)
  49. Difficulty of thawing H9c2 - (reply: 3)
  50. Cryopreservation of MDCK - (reply: 1)
  51. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  52. Problem with Thawing (Freezing) - (reply: 3)
  53. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  54. Extensive MEF death upon thawing - (reply: 1)
  55. Freezing cells - How long can I keep cells at -80C? (reply: 6)
  56. Thaw HEK293 - (reply: 2)
  57. Accidental thawing of frozen cells - (reply: 3)
  58. Low adherence in thawed HUVEC - HUVEC won't attach after thawing (reply: 4)
  59. Cryopreservation of tissue in 50mL centrifuge tube (Urgent) - (reply: 2)
  60. Freezing PCR product??? - (reply: 1)
  61. Freezing Solution - (reply: 1)
  62. Freezing eluted His tag protein - (reply: 5)
  63. To thaw or not to thaw - (reply: 4)
  64. How often do you thaw new cells? - passage number or time? (reply: 7)
  65. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  66. cell cryopreservation after siRNA transfection - (reply: 1)
  67. Storage of Freezing Media - (reply: 2)
  68. Freezing cells- Media or FBS ? - (reply: 2)
  69. problem on removing coverslip for FISH after liquid N2 freezing - when it's still frozen or after thawing? (reply: 1)
  70. Passage/freezing T-cell line calculation problems - (reply: 3)
  71. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
  72. freezing during RNA isolation step - (reply: 1)
  73. Freezing U937 - (reply: 1)
  74. Freezing THP-1 - (reply: 11)
  75. Freezing primers+SYBR green - (reply: 2)
  76. Thawing and Plating - (reply: 4)
  77. Thawing and Plating - (reply: 1)
  78. please help with Freezing primary cells - (reply: 1)
  79. freezing suspended cells - (reply: 7)
  80. Newbie question: Stability of protein lystates - Freeze/thaw cycles affect phosphate groups? (reply: 2)
  81. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  82. Repeat thawing and freezing of antibiotics? - (reply: 9)
  83. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
  84. Freezing cells, quick question - (reply: 1)
  85. freezing after reducing and boiling - (reply: 10)
  86. Do you lose RNA by quick freezing? - (reply: 5)
  87. freezing of E.coli culture prior DNA prep - (reply: 5)
  88. HACAT cells - very low cell viability after thawing cells (reply: 18)
  89. method of thawing microsomes - (reply: 1)
  90. Freeze thawing RNA - (reply: 4)
  91. freezing of digitonin solutions - (reply: 2)
  92. Tips and FAQs about freezing and thawing cells - Abcam (reply: 26)
  93. Effect of freezing on DNA methylation? - (reply: 6)