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Kd value from Km value - (Oct/31/2011 )

I have a enzyme and I know its Km, Vm and Kcat value but I dont know its Kd value. How to drive the Kd value from Km value. I shall be very thankful to you for your kind reply.

best regards


The Km is approximately the KD (dissociation constant) for the substrate


Agree with Bluefunk. Km and Kd are different , but at the same order. Usually, they are measured with different assays. I do not think you can convert Kd from Km.


dissociation constant(Kd) is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its component ions.