Fluorescence at 560 - (Aug/04/2011 )
This is the fluorescence profile Iím getting with my protein bound vs. unbound. Any ideas on what that bump at 560 is?
You forgot to attach the profile...
Can you also provide a baseline profile (i.e. no interaction)
The Lower curve is protein alone. The ligand does not fluoresce. It's not ultra important what it is as my quench flow kinetics make sense and agree with my NMR data. I'm just wondering what that little blip at 560 might be.
the profile is still not attached. we hard to analyze what we can't see.
is 560 the excitation or emission wavelength?
is the blip before or after the peak wavelength?
there could be some quenching or conformational change or ...