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cholestrol determination in blood - (Jan/20/2012 )

can any one tell me about cholestrol determiantion in blood

-eman-

When we measure cholesterol, we extract all the lipids in the cell using a bligh and dyer differential extraction (google it, its simple). In our lab, we use an Iatroscan TLC-FID (thin layer chromatography flame ionization detector), which separates the lipids and when compared to standards, quantifies them by detecting the ions formed when it is burnt).

-Bluefunk-

in vitro assays based on cholesterol oxidase reaction

-Inmost sun-