cholestrol determination in blood - (Jan/20/2012 )
can any one tell me about cholestrol determiantion in blood
When we measure cholesterol, we extract all the lipids in the cell using a bligh and dyer differential extraction (google it, its simple). In our lab, we use an Iatroscan TLC-FID (thin layer chromatography flame ionization detector), which separates the lipids and when compared to standards, quantifies them by detecting the ions formed when it is burnt).
in vitro assays based on cholesterol oxidase reaction