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Suggest best protocol for Adenosine deaminase activity - (Dec/02/2012 )

Hi all!

Can any one suggest me the best method to estimate the activity of adenosine deaminase in the blood.

How we can measure its activity on adenosine ... one way is to know the amount of ammonia being produced,,,

one of the articles I referred did a spec at 630 nm and quantified the ADA activity using Bertholet Method.

But, it did not work good for me ... I got all fluctuated results.. I took care of GLP ... but, I am helpless.


would this help?