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361. mirVana qRT-PCR with the Roche 1.5 LightCycler - (reply: 1)
362. How to trace siRNA in cells - (reply: 4)
363. Glioblastoma cell lines and transfection - stable transfection (reply: 5)
364. DNA:RNA hybrid - is it more stable than DNA:DNA or RNA:RNA (reply: 4)
365. Time course for siRNA inhibition - When is the peak of inhibition after transfection ? (reply: 11)
366. how to use miranda - (reply: 2)
367. shRNA cloning - (reply: 4)
368. Dicer Cleavage - Is there a particular spot? (reply: 2)
369. help with choosing model system for optimization of transfection of siRNA - and best deal for positive control? (reply: 5)
370. Can't repeat a perfect miRNA real-time standard curve - (reply: 2)
371. Does Dox pass the Blood-Brain-barrier? - (reply: 1)
372. siRNA failure - Knock down failure with oligofectamine (reply: 5)
373. How to detect micRNA in some certain tissue ? - (reply: 4)
374. Seeking House keep gene under 200nt - (reply: 5)
375. Confirming SiRNA Knockdown - (reply: 6)
376. shRNA Design - (reply: 2)
377. siRNA duplex length, 27mer? - anyone tested that 27mer is better? (reply: 1)
378. Increased transcripts after RNAi - (reply: 10)
379. Difference between SiRNA and shRNA - (reply: 2)
380. Problem with shRNA stable transfection - (reply: 3)
381. small RNA (100-500bp) - (reply: 1)
382. pSM2C retrovirus vector from Openbiosystems, is it good? - (reply: 5)
383. siRNA transfection of MIN6 cells - (reply: 2)
384. siRNA extraction from plant material - (reply: 5)
385. miRNA target prediction tools - (reply: 2)
386. Control miRNA in experiments? - let-7 or miR-30??? (reply: 2)
387. siRNA purification - (reply: 2)
388. DICER Western Blot - (reply: 5)
389. overexpression of miRNA in a shRNA-vector? - (reply: 1)
390. siRNA Cloning method - (reply: 2)

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