How to detect micRNA in some certain tissue ？ - (Jun/14/2006 )
How to detect micRNA in some certain tissue ？for example mussle tissue. weclcome to discuss this theme.Methods and reference papers are all ok.
My means is how to find microRNA in certain tissues
northern blot or mirvana from ambion are good methods.
microarray or RT-PCR, high though put
I you want to see a find a profile, rye is right on - microarray or a lot of RT-PCR assays. If you have a particular miRNA in mind a couple companies provide assays-on-demand and you can choose which ones you want to look at. you do have to start with your RNA, and make sure the isolation method retains small RNAs or use a total prep method that does not exclude by size.