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amplicon size... when is too small too small? - (Nov/21/2006 )

hi there,

i've designed a nice pair of primers for human insulin receptor which have no secondary structures but the amplicon size is only 67bp.... is this too small? i was originally told that 75 was the smallest i should use?




I work with products at the size around of 65bp, and also found sb conduct real time PCR with ~55bp amplicons