TRAP staining - TRAP staining of osteoclasts in vitro (Aug/12/2008 )
i'm doing some TRAP staining on in vitro osteoclasts and want to know if it's ok if i only fix the cells today with paraformaldehyde and then tomorrow do permeabilization with triton x-100 and then TRAP staining. will this have any bad effects or should i only do it in one day? thanks a bunch!
dana
-danalcat-
It's best to stain ASAP, but if you really can't do it that way, I would fix for 10-15 minutes, wash with Tris-buffered saline (not PBS!) and then store at 4`C overnight in Tris-buffered saline
-JAH-
QUOTE (JAH @ Aug 12 2008, 12:49 PM)
It's best to stain ASAP, but if you really can't do it that way, I would fix for 10-15 minutes, wash with Tris-buffered saline (not PBS!) and then store at 4`C overnight in Tris-buffered saline
sweet! thanks so much!

-danalcat-
QUOTE (danalcat @ Aug 12 2008, 11:32 AM)
QUOTE (JAH @ Aug 12 2008, 12:49 PM)
It's best to stain ASAP, but if you really can't do it that way, I would fix for 10-15 minutes, wash with Tris-buffered saline (not PBS!) and then store at 4`C overnight in Tris-buffered saline
sweet! thanks so much!

Hi
Iam trying to do TRAP stain in osteoclast from RAW cells but dont see any staining. But I am sure there are lots of osteoclasts. Can you please forward me the protocol
Thanks
KBA
-KBA-