can't detect bcl-2 in cytosol - but Bax is there! (Dec/15/2008 )
I did cellular fractionation and managed to detect Bax in the cytosol of my samples, but Bcl-2 is not there. in fact Bcl-2 is in my nuclei fraction. why is that? isn't bcl-2 supposed to be in cytosol?
-Curtis-
Cell Growth Differ. 1994 Apr;5(4):411-7.
The protein product of the oncogene bcl-2 is a component of the nuclear envelope, the endoplasmic reticulum, and the outer mitochondrial membrane.
Hope this helps!
-dtimm-
QUOTE (dtimm @ Dec 16 2008, 07:40 AM)
Cell Growth Differ. 1994 Apr;5(4):411-7.
The protein product of the oncogene bcl-2 is a component of the nuclear envelope, the endoplasmic reticulum, and the outer mitochondrial membrane.
Hope this helps!
The protein product of the oncogene bcl-2 is a component of the nuclear envelope, the endoplasmic reticulum, and the outer mitochondrial membrane.
Hope this helps!
thanks, my supervisor told me the same thing.
I thought some of it floats in cytosol like Bax. I was wrong
-Curtis-