mir30-based shrna cloning-mismatch - (Dec/23/2011 )
I cloned a mir-30 based shrna on a lentiviral vector recently. I submitted for sequencing and found out that there is some mismatches on sense and antisense sequences but not mir30 context and loop sequence.
Any ideas why it is like that and how to avoid it?
It is not clear to me what you mean by "not