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CDNA Related Discussions
  1. cDNA degradation within a week at -20? - (reply: 2)
  2. Amount of RNA for cDNA - (reply: 3)
  3. Primer working with some cDNA samples and not other - (reply: 2)
  4. Standardizing cDNA concentration before doing PCR - (reply: 4)
  5. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
  6. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  7. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  8. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  9. cDNA Library Construction by using kits and Sequencing - (reply: 1)
  10. 3´ RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
  11. OVA cDNA clone? - (reply: 2)
  12. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  13. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  14. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  15. cDNA not enough-variability - (reply: 2)
  16. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
  17. cDNA preparation from degraded RNA - (reply: 2)
  18. non-reproducible PCR results with cDNA as template - (reply: 1)
  19. difference between cDNA and mRNA - (reply: 5)
  20. Concentration of cDNA from unknown quantity of RNA by mistake - (reply: 7)
  21. 100 ng RNA for cDNA synthesis - (reply: 4)
  22. Whole genome amplification from cDNA? - (reply: 4)
  23. Double strand cdna synthysis - (reply: 4)
  24. TA Cloning of ds cDNA - (reply: 6)
  25. Cloning cDNA construct into E.Coli. - (reply: 4)
  26. cDNA amount in qPCR - (reply: 2)
  27. Strange control contamination in cDNA synthesis - (reply: 2)
  28. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  29. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
  30. cDNA test on 1% agarose gel - (reply: 3)
  31. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  32. PCR primer usage (Clonning & cDNA) - (reply: 2)
  33. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  34. Why RNase H treatment after cDNA synthesis - (reply: 2)
  35. cDNA amplification problem - (reply: 4)
  36. get EST/ cDNA accession number for protein - (reply: 1)
  37. difference between CDNA & CDS - (reply: 1)
  38. DNA or cDNA? - (reply: 1)
  39. cDNA sequencing - (reply: 2)
  40. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  41. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  42. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
  43. Primers amplify genomic DNA but not cDNA - (reply: 2)
  44. RT / cDNA synthesis - (reply: 7)
  45. fusion of cDNA expressing protein with gfp - (reply: 1)
  46. cloning of a long gene using cDNA - (reply: 1)
  47. Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
  48. Explain the basic principles of how a cDNA microarray operates? - (reply: 2)
  49. Plasmid vs cDNA amplification by PCR - (reply: 2)
  50. cdna synthesis - (reply: 1)
  51. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  52. cDNA concentration of qPCR - (reply: 1)
  53. cDNA synthesis or RT-PCR - (reply: 3)
  54. PCR on cDNA and SGamplification??? - (reply: 1)
  55. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  56. Maximum length of cDNA from RT reaction - (reply: 6)
  57. cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
  58. Clarifications on cDNA meaning in NCBI + Drosophila Melanogaster quantitative-re - (reply: 2)
  59. Difference between EST and cDNA? - (reply: 4)
  60. cdna library. - (reply: 2)
  61. Measuring of cDNA bands density - (reply: 3)
  62. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
  63. cDNA shelf-life - (reply: 1)
  64. Full length cDNA - (reply: 4)
  65. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  66. PCR using CDNA as template - (reply: 2)
  67. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  68. Glycosilated peptide's cDNA cloning - (reply: 3)
  69. cDNA synthesis + amplification - (reply: 2)
  70. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  71. cDNA clone - (reply: 1)
  72. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  73. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  74. RT-PCR cDNA synthesis - (reply: 6)
  75. problems amplifying from cDNA - (reply: 4)
  76. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  77. cDNA synthesis error - (reply: 2)
  78. PCR of microRNA first strand cDNA - (reply: 1)
  79. iScript BioRad cDNA synthesis yield - (reply: 1)
  80. cDNA synthesis - (reply: 6)
  81. Viral cDNA Library - (reply: 3)
  82. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  83. Single or double-stranded cDNA? - (reply: 2)
  84. copy number calculation from cDNA - (reply: 1)
  85. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  86. total cDNA amplification by PCR - (reply: 2)
  87. cDNA synthesis - (reply: 3)
  88. cDNA contamination, pls help! - (reply: 1)
  89. how to determine the amount of cDNA for qPCR - (reply: 5)
  90. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  91. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
  92. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  93. generating cDNA from human brain RNA - (reply: 1)
  94. cDNA synthesis - (reply: 3)
  95. cDNA storage and real time RT PCR - (reply: 3)
  96. obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
  97. ds cDNA synthesis problem - (reply: 1)
  98. commercially available cDNA constructs - feeling lazy and don't want to make them. (reply: 1)
  99. How to obatin intron sequence from cDNA sequence? - (reply: 1)
  100. cDNA convertion to DNA - (reply: 4)
  101. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
  102. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  103. problem with pcr cloning from mouse cDNA - (reply: 2)
  104. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  105. cDNA as PCR template - (reply: 1)
  106. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
  107. cDNA library construction - I get only one colony (reply: 2)
  108. Cloning from cDNA - the wrong gene?! - (reply: 2)
  109. cDNA for qPCR - Help with basics please (reply: 6)
  110. RACE kits - opinions - What brand do you use to get RACE ready cDNA? (reply: 2)
  111. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  112. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  113. Problem cDNA synthesis/ 2 step RT - (reply: 6)
  114. cDNA concentration higher than original RNA - (reply: 4)
  115. cDNA clone - (reply: 1)
  116. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
  117. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
  118. reverse transcriptase and cDNA - (reply: 1)
  119. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  120. need help cloning cdna of a known protein - (reply: 1)
  121. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  122. minimum amount of cDNA? - molecular biology (reply: 6)
  123. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  124. stupid question on cloning - what part of cDNA is really needed? (reply: 2)
  125. Simple method to determine the size of single-stranded cDNA? - (reply: 3)
  126. Storage of Cy3/Cy5-labled cDNA - (reply: 2)
  127. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
  128. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  129. Amplify cDNA for qRT-PCR? - (reply: 6)
  130. cDNA evaluation and quantification - (reply: 5)
  131. increase the amount of cDNA - (reply: 3)
  132. cDNA 260/280 ratio - (reply: 3)
  133. qPCR and varying cDNA concentrations - (reply: 1)
  134. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
  135. Effects of more cDNA in PCR??? - (reply: 1)
  136. cDNA - (reply: 3)
  137. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  138. Best courier service for international shipping of cDNA? - I need to ship some cDNA from Denmark to the US. (reply: 1)
  139. ds cDNA synthesis kit - one step or two step (reply: 2)
  140. need guidence for cdna library - screen library (reply: 5)
  141. 18S changes with cDNA dilution - (reply: 2)
  142. help for cDNA synthesis - plus RE problem. (reply: 3)
  143. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  144. strategy to isolate cDNA and express protein - (reply: 1)
  145. cDNA spectrophotometric quantification - (reply: 8)
  146. How to find catalytic domain using cDNA - (reply: 2)
  147. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  148. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  149. Maxi Prep cDNA contamination - (reply: 2)
  150. cDNA stability at 4 C - (reply: 4)
  151. cDNA question - (reply: 3)
  152. cDNA cloning problem - (reply: 1)
  153. cDNA versus cRNA for a microarray - (reply: 1)
  154. using cDNA synthesis kit past expiration??? - cDNA synthesis (reply: 2)
  155. How to control cloned cDNA expression level in cells? - (reply: 8)
  156. Identifying cDNA insert - Basic help needed (reply: 2)
  157. Best method to concentrate RNA/cDNA? - (reply: 5)
  158. Is cDNA an ORF? - (reply: 4)
  159. cDNA and RT-PCR - (reply: 8)
  160. cDNA expression - (reply: 3)
  161. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
  162. Elute cDNA from filter paper - (reply: 1)
  163. cDNA to find - (reply: 1)