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CDNA Related Discussions
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How to prepare serial dilutions of RNA or cDNA - (reply: 3)
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cDNA test on 1% agarose gel - (reply: 3)
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can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
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PCR primer usage (Clonning & cDNA) - (reply: 2)
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How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
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Why RNase H treatment after cDNA synthesis - (reply: 2)
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cDNA amplification problem - (reply: 4)
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get EST/ cDNA accession number for protein - (reply: 1)
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difference between CDNA & CDS - (reply: 1)
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DNA or cDNA? - (reply: 1)
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cDNA sequencing - (reply: 2)
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Primer concentration for cDNA synthesis using GSP - (reply: 1)
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cloning from cDNA but got a much shorter PCR product - (reply: 4)
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Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
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Primers amplify genomic DNA but not cDNA - (reply: 2)
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RT / cDNA synthesis - (reply: 7)
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fusion of cDNA expressing protein with gfp - (reply: 1)
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cloning of a long gene using cDNA - (reply: 1)
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Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
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Explain the basic principles of how a cDNA microarray operates? - (reply: 2)
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Plasmid vs cDNA amplification by PCR - (reply: 2)
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cdna synthesis - (reply: 1)
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5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
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cDNA concentration of qPCR - (reply: 1)
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cDNA synthesis or RT-PCR - (reply: 3)
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PCR on cDNA and SGamplification??? - (reply: 1)
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Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
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Maximum length of cDNA from RT reaction - (reply: 6)
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cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
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Clarifications on cDNA meaning in NCBI + Drosophila Melanogaster quantitative-re - (reply: 2)
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Difference between EST and cDNA? - (reply: 4)
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cdna library. - (reply: 2)
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Measuring of cDNA bands density - (reply: 3)
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I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
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cDNA shelf-life - (reply: 1)
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Full length cDNA - (reply: 4)
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Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
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PCR using CDNA as template - (reply: 2)
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RT-PCR primer design for full length cDNA cloning - (reply: 6)
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Glycosilated peptide's cDNA cloning - (reply: 3)
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cDNA synthesis + amplification - (reply: 2)
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To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
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cDNA clone - (reply: 1)
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cDNA for plasmid transformation PLEASE HElP - (reply: 3)
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Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
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RT-PCR cDNA synthesis - (reply: 6)
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problems amplifying from cDNA - (reply: 4)
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Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
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cDNA synthesis error - (reply: 2)
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PCR of microRNA first strand cDNA - (reply: 1)
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iScript BioRad cDNA synthesis yield - (reply: 1)
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cDNA synthesis - (reply: 6)
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Viral cDNA Library - (reply: 3)
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First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
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Single or double-stranded cDNA? - (reply: 2)
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copy number calculation from cDNA - (reply: 1)
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Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
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total cDNA amplification by PCR - (reply: 2)
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cDNA synthesis - (reply: 3)
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cDNA contamination, pls help! - (reply: 1)
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how to determine the amount of cDNA for qPCR - (reply: 5)
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About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
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conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
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Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
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generating cDNA from human brain RNA - (reply: 1)
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cDNA synthesis - (reply: 3)
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cDNA storage and real time RT PCR - (reply: 3)
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obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
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ds cDNA synthesis problem - (reply: 1)
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commercially available cDNA constructs - feeling lazy and don't want to make them. (reply: 1)
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How to obatin intron sequence from cDNA sequence? - (reply: 1)
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cDNA convertion to DNA - (reply: 4)
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cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
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Identifying unknown cDNA inserts from restriction digests - (reply: 2)
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problem with pcr cloning from mouse cDNA - (reply: 2)
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PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
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cDNA as PCR template - (reply: 1)
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Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
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cDNA library construction - I get only one colony (reply: 2)
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Cloning from cDNA - the wrong gene?! - (reply: 2)
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cDNA for qPCR - Help with basics please (reply: 6)
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RACE kits - opinions - What brand do you use to get RACE ready cDNA? (reply: 2)
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first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
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How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
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Problem cDNA synthesis/ 2 step RT - (reply: 6)
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cDNA concentration higher than original RNA - (reply: 4)
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cDNA clone - (reply: 1)
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How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
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isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
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reverse transcriptase and cDNA - (reply: 1)
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Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
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need help cloning cdna of a known protein - (reply: 1)
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Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
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minimum amount of cDNA? - molecular biology (reply: 6)
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problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
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stupid question on cloning - what part of cDNA is really needed? (reply: 2)
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Simple method to determine the size of single-stranded cDNA? - (reply: 3)
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Storage of Cy3/Cy5-labled cDNA - (reply: 2)
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help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
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same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
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Amplify cDNA for qRT-PCR? - (reply: 6)
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cDNA evaluation and quantification - (reply: 5)
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increase the amount of cDNA - (reply: 3)
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cDNA 260/280 ratio - (reply: 3)
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qPCR and varying cDNA concentrations - (reply: 1)
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Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
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Effects of more cDNA in PCR??? - (reply: 1)
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cDNA - (reply: 3)
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RT-PCR primer dimers and cDNA degradation - (reply: 2)
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Best courier service for international shipping of cDNA? - I need to ship some cDNA from Denmark to the US. (reply: 1)
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ds cDNA synthesis kit - one step or two step (reply: 2)
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need guidence for cdna library - screen library (reply: 5)
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18S changes with cDNA dilution - (reply: 2)
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help for cDNA synthesis - plus RE problem. (reply: 3)
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cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
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strategy to isolate cDNA and express protein - (reply: 1)
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cDNA spectrophotometric quantification - (reply: 8)
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How to find catalytic domain using cDNA - (reply: 2)
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Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
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gDNA or cDNA amplification in RT-PCR - (reply: 3)
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Maxi Prep cDNA contamination - (reply: 2)
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cDNA stability at 4 C - (reply: 4)
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cDNA question - (reply: 3)
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cDNA cloning problem - (reply: 1)
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cDNA versus cRNA for a microarray - (reply: 1)
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using cDNA synthesis kit past expiration??? - cDNA synthesis (reply: 2)
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How to control cloned cDNA expression level in cells? - (reply: 8)
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Identifying cDNA insert - Basic help needed (reply: 2)
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Best method to concentrate RNA/cDNA? - (reply: 5)
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Is cDNA an ORF? - (reply: 4)
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cDNA and RT-PCR - (reply: 8)
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cDNA expression - (reply: 3)
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cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
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Elute cDNA from filter paper - (reply: 1)
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cDNA to find - (reply: 1)