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RT Related Discussions
  1. need help setting up reverse transcriptase qPCR - (reply: 3)
  2. could you help me with my stem loop RT primer? - (reply: 1)
  3. Need no RT control for all genes? - (reply: 1)
  4. Question about the RT PCR - (reply: 3)
  5. Reverse transcription 101 question! - (reply: 5)
  6. skip 65 degrees protocol of Reverse transcription - (reply: 4)
  7. RNA concentration to start RT reaction - (reply: 1)
  8. Random vs oligo primer in preamplification RT - (reply: 1)
  9. A question for Reverse transcription experts - (reply: 5)
  10. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  11. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
  12. Strange RT to qPCR results with new kit - (reply: 5)
  13. Plasmid DNA at RT for a few days - (reply: 4)
  14. What is your preferred Reverse Transcriptase? - (reply: 4)
  15. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  16. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
  17. Error rate of commecial reverse transcriptase - (reply: 1)
  18. RT / cDNA synthesis - (reply: 7)
  19. Maximum length of cDNA from RT reaction - (reply: 6)
  20. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  21. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  22. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  23. Stability of DNA at RT - (reply: 1)
  24. Splice varient quantification in RT real time PCR? - (reply: 1)
  25. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  26. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  27. How long can diluted primary antibdoy remain effective at RT? - (reply: 7)
  28. cDNA storage and real time RT PCR - (reply: 3)
  29. Anitbiotic shelf life in liquid media at RT - (reply: 2)
  30. Question about RT... - (reply: 1)
  31. RT PCR for known snps detection? - SNPs detection (reply: 1)
  32. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
  33. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  34. use of housekeeping gene in RT PCR chIP - (reply: 3)
  35. Forgot to stop reverse transcription reaction - (reply: 4)
  36. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  37. Dumb Reverse Transcription PCR Question - (reply: 2)
  38. Reverse transcription PCR - (reply: 1)
  39. Embryo preservation for IHC at RT?? - (reply: 1)
  40. silly control question - No RT controls (reply: 2)
  41. Relative real time RT PCR - (reply: 2)
  42. How to do PCR to detect mRNA without RT? - (reply: 2)
  43. multiplexing taqman miRNA reverse transcription - (reply: 2)
  44. Problem cDNA synthesis/ 2 step RT - (reply: 6)
  45. Reverse transcription question - Volume for RT reaction (reply: 3)
  46. Antibody stability if left out at RT over Xmas break? - (reply: 5)
  47. Does RT step smplify RNA - (reply: 2)
  48. reverse transcriptase and cDNA - (reply: 1)
  49. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  50. The perfect RT??? which control? - (reply: 4)
  51. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  52. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  53. heating rna before rt reaction? - (reply: 1)
  54. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
  55. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 4)
  56. RT qPCR Newbie, Weird Results - (reply: 1)
  57. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  58. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
  59. RNA reverse transcription riddle - (reply: 6)
  60. amplification in negative control in RT PCR - (reply: 2)
  61. Reverse Transcription - (reply: 3)
  62. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)