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Protocols
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Test Tube for Ion Exchange Chromatography
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
The purpose of the test tube is to find proper binding conditions such as pH and resin type, of the target protein (or contaminating proteins) to IEX (ionic exchange) resins. Once you know the best pH binding conditions, a second test tube at different salt concentrations will provide information about washing and elution conditions.
http://wolfson.huji.ac.il/purification/Protocols/T...
Added: Sat Feb 21 2009, Hits: 898, Reviews: 0 Write review Cached -
Tips for acid elution of peptides
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Very useful tips
http://www.its.caltech.edu/%7Ebjorker/acid_elution...
Added: Wed Aug 14 2002, Hits: 756, Reviews: 0 Write review -
Whole Cell Protein Extracts
(Morrisey Lab, University of Pennsylvania)
This is for both cytoplasmic and nuclear proteins.
http://www.uphs.upenn.edu/mcrc/morrisey/cellextrac...
Added: Mon Oct 14 2002, Hits: 2204, Reviews: 0 Write review Cached
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