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Protocols
- Small scale His-Tag fusion protein purification under denaturative conditions (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
Describes two protein purification procedures. Procedure-I, where the protein is extracted from the bacterial pellet in the presence of denaturating agents and purified on Ni2+ beads; or Procedure-II where the protein is solubilized from partially clean or clean IB before purification on Ni2+ beads. The second procedure although more laborious, can help to reduce the background of protein contaminants in the final purification step.
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 5051, Reviews: 0 Write review Cached - Small scale His-Tag fusion protein purification under nature conditions (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 5407, Reviews: 0 Write review Cached -
His-tag protein purification using Ni-NTA magnetic beads
(The Protein Expression and Purification Facility, EMBL)
http://www.embl-heidelberg.de/ExternalInfo/geerlof...
Added: Sat Sep 21 2002, Hits: 4257, Reviews: 0 Write review Cached -
His-Tagged Protein Purification
(Chen's Lab)
http://www.biochem.ucl.ac.uk/~chen/protocols/hista...
Added: Tue May 14 2002, Hits: 7160, Reviews: 0 Write review -
Preparation of affinity resins
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Coating of CNBr-activated resins sepharose 4B with antigen
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Sun Mar 01 2009, Hits: 1123, Reviews: 0 Write review Cached -
Production of N-His6-tagged TEV protease
(Dr. Arie Geerlof)
http://www.embl-hamburg.de/~geerlof/protocols/TEV-...
Added: Sun Oct 25 2009, Hits: 1148, Reviews: 0 Write review -
Protein purification using Ni-NTA resin
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Proteins with a 6xHis tag can be purified through Nichel nitrilotriacetic (Ni-NTA) resin.
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Sun Mar 01 2009, Hits: 4395, Reviews: 0 Write review Cached -
Purification of 6X HIS Proteins
(Dr. Ian Chin-Sang)
Purification of 6XHIS proteins with imidazole cell extraction buffer.
http://lab.methodmint.com/methods/1444/
Added: Sat Feb 18 2012, Hits: 242, Reviews: 0 Write review -
Purification of His-tagged proteins
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Martin_purif...
Added: Wed Aug 14 2002, Hits: 6139, Reviews: 0 Write review -
Purification of Histagged Protein
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
This protocol is used to purify histagged TfR expressed using the baculo virus expression system.It consist of three steps: (1) buffer exchange using the Amicon spiral concentrator; (2) batch/column Ni-NTA purification; (3) a gel filtration purification. Normally, the purification yield I obtain from the media received by Peter Snow is 4-10 mg/liter.
http://www.its.caltech.edu/%7Ebjorker/TFR_(histagg...
Added: Wed Aug 14 2002, Hits: 4909, Reviews: 0 Write review