Protocols
- Small Scale GST-Tag Purification Under Nature Conditions
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 3086, Reviews: 0 Write review Cached -
Denaturing purification of insoluble bacterial GST-fusion Proteins
(Whitelabs.org)
http://www.whitelabs.org/Lab%20Protocols/bacterial...
Added: Fri Nov 06 2009, Hits: 1312, Reviews: 0 Write review Cached -
Elution of GST-Fusion Proteins from Glutathione Agarose
(Millipore)
http://www.millipore.com/techpublications/tech1/mc...
Added: Sat Feb 21 2009, Hits: 1999, Reviews: 0 Write review -
GST Fusion Protein Preparation
(Vesicle Trafficking)
Detailed protocol for GST protein purification.
http://cmgm.stanford.edu/~vesicle/protocols/gst_pr...
Added: Tue May 14 2002, Hits: 4503, Reviews: 0 Write review Cached -
GST Fusion Protein Purification
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
Starts from making contructs, protein expression and GST fusion protein purification using gluthione sepharose beads
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Thu Oct 03 2002, Hits: 4501, Reviews: 0 Write review Cached -
GST Fusion Protein Purification from Yeast
(Henrik G. Dohlman, The University of North Carolina Chapel Hill)
http://www.med.unc.edu/~hdohlman/gst.html
Added: Sun Aug 18 2002, Hits: 1097, Reviews: 0 Write review Cached -
GST-Fusion Protein Purification
(Chen's Lab)
http://www.biochem.ucl.ac.uk/~chen/protocols/gstpu...
Added: Tue May 14 2002, Hits: 2984, Reviews: 0 Write review -
Purification of GST Fused Proteins
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 298, Reviews: 0 Write review Cached - Purification of GST Fused Proteins (Contributed by Chia Jin Ngee)
Many people have vented out frustration over insoluble GST-fused proteins. This is a protocol for enzymatically active soluble GST-fused proteins. All GST-fused proteins are rendered soluble with this technique though enzyme activitiy can range from 30-90%.
Added: Tue May 14 2002, Reviews: 0 Write review -
Purification of GST-Fusion Proteins from E. coli.
(Mullins Lab, UCSF)
http://mullinslab.ucsf.edu/protocols/html/GST_fusi...
Added: Sat Feb 21 2009, Hits: 1076, Reviews: 0 Write review Cached