Blood and Erythrocyte Fractionation
(David R. Caprette, Rice University )
In this protocol, red blood cells will be isolated from whole blood and washed free of contaminating plasma proteins. The cells will then be lysed (ruptured) to release the cytoplasmic proteins from the membranes, and the membranes will be separated from the dilute cytoplasm using a series of wash steps. Aliquots (samples) of critical steps in the cell fractionation process will be taken for analysis, to monitor the effectiveness of the separation.
Added: Sun Sep 29 2002, Hits: 2127, Reviews: 0
Preparation of Brain Membrane Fractions for Western Blot Analysis
This is a standard protocol adapted from Rogers et al., 1991.
Added: Tue May 14 2002, Hits: 3251, Reviews: 0