Subcategories
Protocols
- Bradford Assay (Kitto Lab, The University of Texas at Austin)
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Sat Mar 14 2009, Hits: 2923, Reviews: 0 Write review Cached -
Comparison of Different Protein Determination Methods (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
Very nice side by side comparisons of various methods
http://wolfson.huji.ac.il/purification/Protocols/C...
Added: Tue Feb 17 2009, Hits: 2804, Reviews: 0 Write review Cached - Protein Assays (David R. Caprette, Rice University)
a list of assays for the determination of protein concentration in a solution. Procedural details, equipment requirements, and references are outlined in the individual assay documents. Highly recommended.
http://www.ruf.rice.edu/~bioslabs/methods/protein/...
Added: Tue Feb 17 2009, Hits: 2660, Reviews: 0 Write review Cached -
Biuret Protein Assay
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/appds/appd-g.html...
Added: Tue May 14 2002, Hits: 1955, Reviews: 0 Write review Cached -
Biuret Protein Assay
(David R. Caprette, Rice University)
Biuret protein assay is based on the principle that under alkaline conditions substances containing two or more peptide bonds form a purple complex with copper salts in the reagent.
http://www.ruf.rice.edu/~bioslabs/methods/protein/...
Added: Tue Feb 17 2009, Hits: 1470, Reviews: 0 Write review Cached -
Phosphoamino Acid Analysis of non-TCA Precipitable Proteins
(Woodgett Lab)
http://kinase.uhnres.utoronto.ca/methods/PAA.html
Added: Tue May 14 2002, Hits: 755, Reviews: 0 Write review Cached -
Protein Concentration of Laemmli Gel Samples
(Woodgett Lab)
http://kinase.uhnres.utoronto.ca/methods/TCA.html
Added: Tue May 14 2002, Hits: 1559, Reviews: 0 Write review Cached -
Protein Quantitation with Spectrophotometer
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sat Nov 02 2002, Hits: 758, Reviews: 0 Write review Cached - Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups (Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg Bulaj, Dept. of Biology, University of Utah)
This is the standard 揈llman抯 Test?for the determination of free thiols. It works well for small peptides and proteins synthesized using standard solid phase synthetic methods.
Added: Tue May 14 2002, Reviews: 0 Write review -
Semi-Quantitative Measurement of Proteins by Dot Blotting
(Springer Lab, Harvard University)
Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibody against it
http://labs.idi.harvard.edu/springer/uploads/Proto...
Added: Wed Aug 14 2002, Hits: 961, Reviews: 1 Read reviews Write review Cached -
Semi-Quantitative Measurement of Proteins by Dot Blotting
(Springer Lab, Harvard University)
Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibody against it.
http://labs.idi.harvard.edu/springer/protocols/jun...
Added: Sun Oct 25 2009, Hits: 334, Reviews: 0 Write review Cached -
UV Absorbance Protein Determination
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
The advantages of this method are that it is simple, and the sample is recoverable. The method has some disadvantages, including interference from other chromophores, and the specific absorption value for a given protein must be determined.
http://wolfson.huji.ac.il/purification/Protocols/O...
Added: Tue Feb 17 2009, Hits: 1732, Reviews: 0 Write review Cached