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Protocols
- Protein Expression and Purification Protocol (Sosnick Group, University of Chicago)
From plasmid to protein, the protocol offers detailed guide to this whole procedure.
http://sosnick.uchicago.edu/Protein_Express&Purify...
Added: Sun Mar 28 2004, Hits: 7014, Reviews: 0 Write review Cached -
Detection of Recombinant Protein by ELISA
(Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation)
Direct ELISA method of detection of recombinant protein in bacteria
http://omrf.ouhsc.edu/~frank/ELISA.html
Added: Thu Sep 01 2005, Hits: 4070, Reviews: 0 Write review Cached -
Expression and purification of a P-ATPase in E. coli
(Biological Procedures Online )
Expression of a prokaryotic P-type ATPase in E. coli plasma membranes and purification by Ni2+-affinity chromatography
http://www.biologicalprocedures.com/bpo/arts/1/9/m...
Added: Wed Sep 25 2002, Hits: 2383, Reviews: 0 Write review Cached -
Insect Cell Culture and Baculovirus Infections
(Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
Insect cell culture, freezing, thawing and transfection.
http://omrf.ouhsc.edu/~frank/BACULPRO.html
Added: Mon Dec 19 2005, Hits: 1530, Reviews: 0 Write review Cached -
Isolation of proteins from inclusion bodies
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Describes protein expression and purification
http://www.its.caltech.edu/%7Ebjorker/Isolation%20...
Added: Wed Aug 14 2002, Hits: 3202, Reviews: 0 Write review - Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase (Hikmet Geckil's Lab)
This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Enzyme recoveries up to 3-fold with respect to sonication can be achieved with this system.
Added: Wed Oct 22 2003, Reviews: 0 Write review -
Protein Purification Protocols
(Lamond Lab, University of Dundee)
The following protocols are provided: - Bugbuster to break open bacterial cells
- Purifying His-tagged proteins
- Purifying GST-tagged proteins
http://www.lamondlab.com/LTprotBacteria.htm
Added: Thu May 08 2003, Hits: 2250, Reviews: 0 Write review Cached -
Purification Tricks for Recombinant Proteins
(Dr. Wilbur H. (Bill) Campbell, Michigan Technological University)
Since purification of a protein can be a complex and time-consuming process, it is often possible to add a DNA sequence to the cloned protein's code which will append a small peptide or even a small protein for facilitating the purification of the recombinant protein after it is expressed...
http://www.bio.mtu.edu/campbell/purifica.htm
Added: Sun Jun 20 2004, Hits: 2856, Reviews: 0 Write review Cached -
Recombinant His-6 RII Purification
(Susan Taylor, University of California at San Diego)
Detailed protocol with typical yield approximately 15 mg per liter of cells
http://pkr.sdsc.edu/html/pk_protocols/h6_rii_proto...
Added: Tue Aug 13 2002, Hits: 624, Reviews: 0 Write review Cached -
Recombinant His-6-Cat Purification
(Susan Taylor, University of California at San Diego)
Use this protocol for most His-6-Proteins including GFP. Typical yield approximately 15 mg per liter of cells
http://pkr.sdsc.edu/html/pk_protocols/h6_cat_proto...
Added: Tue Aug 13 2002, Hits: 509, Reviews: 0 Write review Cached