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• Protein Purification

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Protocols

• Protein Expression and Purification Protocol (Sosnick Group, University of Chicago)
  From plasmid to protein, the protocol offers detailed guide to this whole procedure.
  http://sosnick.uchicago.edu/Protein_Express&Purify...
  Added: Sun Mar 28 2004, Hits: 890, Reviews: 0  Write review   Cached
• Cleavage of His-Tagged Proteins with Thrombin cleavage sites (Chazin Lab, Center for Structural Biology, Vanderbilt University)
  General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites. Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly.
  http://structbio.vanderbilt.edu/chazin/wisdom/labp...
  Added: Sat Oct 18 2003, Hits: 1120, Reviews: 0  Write review   Cached
• Expression and purification of a P-ATPase in E. coli (Biological Procedures Online )
  Expression of a prokaryotic P-type ATPase in E. coli plasma membranes and purification by Ni2+-affinity chromatography
  http://www.biologicalprocedures.com/bpo/arts/1/9/m...
  Added: Wed Sep 25 2002, Hits: 567, Reviews: 0  Write review   Cached
• GST Pull-Downs from Drosophila Tissue Culture Cells (BioProtocol)
  Describes preparing, binding to glutathione-agarose beads, pull-down experiment
  http://bioprotocol.bio.com/protocolstools/protocol...
  Added: Thu Oct 03 2002, Hits: 367, Reviews: 0  Write review   Cached
• Isolation of proteins from inclusion bodies (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
  Describes protein expression and purification
  http://www.its.caltech.edu/%7Ebjorker/Isolation%20...
  Added: Wed Aug 14 2002, Hits: 1677, Reviews: 0  Write review  
• Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase (Hikmet Geckil's Lab)
  This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Enzyme recoveries up to 3-fold with respect to sonication can be achieved with this system.
  Added: Wed Oct 22 2003, Reviews: 0  Write review  
• Protein Purification Protocols (Lamond Lab, University of Dundee)
  The following protocols are provided: - Bugbuster to break open bacterial cells
  - Purifying His-tagged proteins
  - Purifying GST-tagged proteins
  
  http://www.lamondlab.com/LTprotBacteria.htm
  Added: Thu May 08 2003, Hits: 749, Reviews: 0  Write review   Cached
• Recombinant His-6 RII Purification (Susan Taylor, University of California at San Diego)
  Detailed protocol with typical yield approximately 15 mg per liter of cells
  http://pkr.sdsc.edu/html/pk_protocols/h6_rii_proto...
  Added: Tue Aug 13 2002, Hits: 269, Reviews: 0  Write review   Cached
• Recombinant His-6-Cat Purification (Susan Taylor, University of California at San Diego)
  Use this protocol for most His-6-Proteins including GFP. Typical yield approximately 15 mg per liter of cells
  http://pkr.sdsc.edu/html/pk_protocols/h6_cat_proto...
  Added: Tue Aug 13 2002, Hits: 214, Reviews: 0  Write review   Cached
• Recombinant PKA Catalytic Subunit (Susan Taylor, University of California at San Diego)
  Step by step protocol on recombinant catalytic subunit preparation (cAMP-dependent Protein Kinase A).
  http://pkr.sdsc.edu/html/pk_protocols/c_prep_proto...
  Added: Tue Aug 13 2002, Hits: 95, Reviews: 0  Write review   Cached
• T-Antigen (SV40 T-ag ) Expression and Purification (Chazin Lab, Center for Structural Biology, Vanderbilt University )
  Express and purify T antigen GST-fusion protein. GST-T antigen has a tendency to form inclusion bodies. Several precautions have to be taken, both during expression and in the purification process to avoid aggregation.
  http://structbio.vanderbilt.edu/chazin/wisdom/labp...
  Added: Fri Oct 17 2003, Hits: 106, Reviews: 0  Write review   Cached