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Protocols

• Protein A Purification of Antibody (Contributed by Nanci Donacki)
  Protein A Purification of Antibody - MAPS II System
  Added: Tue May 14 2002, Reviews: 0  Write review  
• Protein G Purification of Antibodies (Contributed by Nanci Donacki)
  Added: Tue May 14 2002, Reviews: 0  Write review  
• Purification of Antiserum or Ascites by Protein A/G Chromatography (Upstate)
  General protocol including Guidelines for Choosing Protein A Agarose or Protein G Agarose, Preparation of a Protein A Agarose or Protein G Agarose Affinity Column, Pouring the Protein A/G Affinity Column, Affinity Chromatography Using Protein A/G Agarose.
  http://www.upstate.com/misc/protocols.q.prot.e.pur...
  Added: Tue Feb 24 2004, Hits: 3470, Reviews: 0  Write review   Cached
• Affinity purification of anti-peptide antibodies (Craig Pikaard's Laboratory at Washington University)
  Affinity purification of chicken or rabbit anti-peptide antibodies
  http://www.biology.wustl.edu/pikaard/PDFs%20and%20...
  Added: Tue Mar 03 2009, Hits: 2107, Reviews: 0  Write review  
• Affinity Purification of Antibodies (Vesicle Trafficking)
  http://cmgm.stanford.edu/~vesicle/protocols/affini...
  Added: Tue May 14 2002, Hits: 3127, Reviews: 0  Write review   Cached
• Affinity Purification of Antibodies (XMMR)
  protocol in great detail, including column preparation, wash and more...
  http://www.xenbase.org/xenbase/original/WWW/Marker...
  Added: Tue May 14 2002, Hits: 1389, Reviews: 0  Write review   Cached
• Affinity Purification of Antibodies from Crude Serum (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 811, Reviews: 0  Write review   Cached
• Affinity Purification of Antibodies from Serum (Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
  http://www.cooperlab.wustl.edu/LabMethodsReagentsO...
  Added: Sat Feb 21 2009, Hits: 2395, Reviews: 0  Write review   Cached
• Anbibody Purification by by Affinity Chromatography (Mullins Lab, UCSF)
  http://mullinslab.ucsf.edu/protocols/html/ANTIBODY...
  Added: Sat Feb 21 2009, Hits: 1255, Reviews: 0  Write review   Cached
• Antibody Purification (Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
  Ammonium sulphate precipitation is used to purify a protein (in this case an immunoglobulin) from a big volume of liquid phase.
  http://www.icp.ucl.ac.be/mexp/vipg_pur.html
  Added: Fri Mar 26 2004, Hits: 2147, Reviews: 0  Write review   Cached
• Antibody Purification (Mike A. Dyer)
  This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.
  http://axon.med.harvard.edu/~cepko/protocol/mike/A...
  Added: Tue May 14 2002, Hits: 2034, Reviews: 0  Write review   Cached
• Antibody Purification on Protein G Column (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
  Protein G rather than Protein A is the column of choice for purifying mouse IgGs from ascites, because mouse IgG1 binds much (much!) better to Protein G, and most mouse mAbs turn out to be IgG1. However, Protein G cannot be used to purify from tissue culture supernatants containing fetal calf serum, because it has a very high affinity for the bovine IgGs in the serum.
  http://www.its.caltech.edu/%7Ebjorker/Antibody_pur...
  Added: Wed Aug 14 2002, Hits: 2571, Reviews: 0  Write review  
• Antibody Purification Protocol (Immunology Applications Core Facility, University of Chicago)
  Antibody purification is possible because the cepharose beads are conjugated with protein which has an affinity under certain conditions to immunoglobulins. This is a basic protocol for antibody purification either by Protein A or Protein G.
  http://iacf.bsd.uchicago.edu/MabHome/AbPurify.htm
  Added: Mon Apr 19 2004, Hits: 313, Reviews: 0  Write review   Cached
• Column Purification of mAB (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
  The FcRn column is usually used for "functional" purification of IgG's, i.e. by binding at pH 6 and eluting at pH 8. The procedure described here was developed to separate anti-FcRn antibodies from contaminating IgG's present in the ascites. Therefore binding is done at pH 8.5 to prevent the FcRn on the column from binding to the Fc portion of the IgGs in the sample; only IgG's specific for FcRn should be able to bind, as in a typical affinity purification. They are then eluted at pH 2.5.
  http://www.its.caltech.edu/%7Ebjorker/Anti-FcRn_mA...
  Added: Wed Aug 14 2002, Hits: 1447, Reviews: 0  Write review  
• Coupling Antibodies to Protein A/G (Breeden Lab)
  http://www.fhcrc.org/labs/breeden/Methods/coupling...
  Added: Tue May 14 2002, Hits: 1033, Reviews: 0  Write review   Cached
• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
  http://mitchison.med.harvard.edu/protocols/ab3.htm...
  Added: Tue May 14 2002, Hits: 944, Reviews: 0  Write review   Cached
• Making Affinity Columns (XMMR)
  http://www.xenbase.org/xenbase/original/WWW/Marker...
  Added: Tue May 14 2002, Hits: 528, Reviews: 0  Write review   Cached
• Purification of Anti Peptide Antibody (Mitshison Lab)
  
  http://mitchison.med.harvard.edu/protocols/ab4.htm...
  Added: Tue May 14 2002, Hits: 1094, Reviews: 0  Write review   Cached
• Purification of mAb (IgG) (Springer Lab, Harvard University)
  Ammonium sulfate cut and purification of Abs by protein A column
  http://www.cbrinstitute.org/labs/springer/protocol...
  Added: Wed Aug 14 2002, Hits: 1481, Reviews: 0  Write review   Cached
• Stripping Antibodies from a Cell or Tissue Samples (The Molecular Probes Technology Network)
  On occasion one may have performed an antibody labeling experiment on very rare cell or tissue samples. The antibodies provided very poor labeling with very high background. Is it possible to remove these poorly labeling antibodies (primaries and secondaries) and relabel the sample?
  http://molecularprobestechnologynetwork.community....
  Added: Thu Oct 14 2010, Hits: 188, Reviews: 0  Write review   Cached