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Protocols
- Protein A Purification of Antibody (Contributed by Nanci Donacki)
Protein A Purification of Antibody - MAPS II System
Added: Tue May 14 2002, Reviews: 0 Write review - Protein G Purification of Antibodies (Contributed by Nanci Donacki)
Added: Tue May 14 2002, Reviews: 0 Write review - Purification of Antiserum or Ascites by Protein A/G Chromatography (Upstate)
General protocol including Guidelines for Choosing Protein A Agarose or Protein G Agarose, Preparation of a Protein A Agarose or Protein G Agarose Affinity Column, Pouring the Protein A/G Affinity Column, Affinity Chromatography Using Protein A/G Agarose.
http://www.millipore.com/techpublications/tech1/mc...
Added: Tue Feb 24 2004, Hits: 5837, Reviews: 0 Write review Cached -
Affinity Purification of Antibodies
(Vesicle Trafficking)
http://cmgm.stanford.edu/~vesicle/protocols/affini...
Added: Tue May 14 2002, Hits: 7060, Reviews: 0 Write review Cached -
Affinity Purification of Antibodies
(XMMR)
protocol in great detail, including column preparation, wash and more...
http://www.xenbase.org/xmmr/Marker_pages/methods_p...
Added: Tue May 14 2002, Hits: 5763, Reviews: 0 Write review Cached -
Affinity Purification of Antibodies from Crude Serum
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 4149, Reviews: 0 Write review Cached -
Antibody Purification
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Ammonium sulphate precipitation is used to purify a protein (in this case an immunoglobulin) from a big volume of liquid phase.
http://www.icp.ucl.ac.be/mexp/vipg_pur.html
Added: Fri Mar 26 2004, Hits: 6044, Reviews: 0 Write review Cached -
Antibody Purification
(Mike A. Dyer)
This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.
http://axon.med.harvard.edu/~cepko/protocol/mike/A...
Added: Tue May 14 2002, Hits: 4588, Reviews: 0 Write review Cached -
Antibody Purification on Protein G Column
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Protein G rather than Protein A is the column of choice for purifying mouse IgGs from ascites, because mouse IgG1 binds much (much!) better to Protein G, and most mouse mAbs turn out to be IgG1. However, Protein G cannot be used to purify from tissue culture supernatants containing fetal calf serum, because it has a very high affinity for the bovine IgGs in the serum.
http://www.its.caltech.edu/%7Ebjorker/Antibody_pur...
Added: Wed Aug 14 2002, Hits: 4731, Reviews: 0 Write review -
Antibody Purification Protocol
(Immunology Applications Core Facility, University of Chicago)
Antibody purification is possible because the cepharose beads are conjugated with protein which has an affinity under certain conditions to immunoglobulins. This is a basic protocol for antibody purification either by Protein A or Protein G.
http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_F...
Added: Mon Apr 19 2004, Hits: 5432, Reviews: 0 Write review Cached