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Protocols
- Protein Purification Protocols (The Protein Expression and Purification Facility, EMBL)
A collection of related protocols for protein purification
http://www.embl-heidelberg.de/ExternalInfo/geerlof...
Added: Sat Sep 21 2002, Hits: 8183, Reviews: 0 Write review Cached -
Purification of Proteins (NCBI)
Proteins can Be purified according to solubility, size, charge, and binding affinity. This book charpter gives background info on various protein purification methods.
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv....
Added: Sat Jun 12 2004, Hits: 4246, Reviews: 0 Write review Cached -
RNA and protein extraction from the same tissue
(Ivan J Delgado)
http://ivaan.com/protocols/139.html
Added: Sun Mar 01 2009, Hits: 2236, Reviews: 0 Write review Cached -
Green Fluorescent Protein Isolation
(Gary J. Lindquester)
http://glindquester.biology.rhodes.edu/molbiol/pro...
Added: Tue May 14 2002, Hits: 318, Reviews: 0 Write review Cached -
HeLa Cell Nuclei Preparation
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
Prepare nuclear extract from HeLa cell
http://qcom.etsu.edu/biochem/protocols/hela.htm
Added: Tue May 14 2002, Hits: 366, Reviews: 0 Write review Cached -
Isolation of Protein from Tissue
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
Nice formated protocol on protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Thu Oct 03 2002, Hits: 4717, Reviews: 0 Write review Cached -
Making Conductivity Curves
(Waters Lab)
For measuring protein conductivity during protein purification
http://www.molbio.princeton.edu/labs/waters/conduc...
Added: Tue May 14 2002, Hits: 99, Reviews: 0 Write review Cached -
PI-PLC Purification
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
PI-PLC purification of recombinant protein.
http://www.its.caltech.edu/%7Ebjorker/PIPLC_purifi...
Added: Wed Aug 14 2002, Hits: 482, Reviews: 0 Write review -
Preparation of Tonsil Lysate
(Springer Lab, Harvard University)
The protocol describes how to prepare human tonsil lysate for use in purification of ICAM-1, LFA-1, and PNAd.
http://www.cbrinstitute.org/labs/springer/protocol...
Added: Wed Aug 14 2002, Hits: 294, Reviews: 0 Write review Cached -
Protein Extraction
(Frank Bottone Jr.)
http://www.unc.edu/~fbottone/protocol/protein.html
Added: Tue May 14 2002, Hits: 7025, Reviews: 0 Write review Cached -
Protein Extraction from Tissues
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Mon Sep 23 2002, Hits: 5670, Reviews: 0 Write review Cached -
Protein Isolation Protocols
(Lamond Lab, University of Dundee)
Some protein isolation methods:
- Cajal body isolation
- Nucleolar isolation
- Chromatin isolation
http://www.lamondlab.com/LTprotPurification.htm
Added: Thu May 08 2003, Hits: 582, Reviews: 0 Write review Cached -
Protein Isolation using Trizol
(Frank Bottone Jr.)
Simultaneous extract DNA and protein.
http://www.unc.edu/~fbottone/protocol/ptrizol.html
Added: Tue May 14 2002, Hits: 1736, Reviews: 2 Read reviews Write review Cached -
Protein Purification
(Michael Blaber)
Introduction to protein purification method: Initial Steps of fractionation of protein
http://wine1.sb.fsu.edu/bch5425/lect29/lect29.htm
Added: Tue May 14 2002, Hits: 1415, Reviews: 0 Write review Cached -
Protein Purification
(Michael Blaber)
Guide to protein purification method: Ion exchange elutions, dialysis, concentration
http://wine1.sb.fsu.edu/bch5425/lect30/lect30.htm
Added: Tue May 14 2002, Hits: 1081, Reviews: 0 Write review Cached -
Protein Purification
(Michael Blaber)
Introduction to protein purification methd: gel filtration, affinity and hydrophobic resins; preparation of resin, plumbing
http://wine1.sb.fsu.edu/bch5425/lect31/lect31.htm
Added: Tue May 14 2002, Hits: 679, Reviews: 0 Write review Cached -
Protein Purification on 1G3 Column
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/1G3_Column-s...
Added: Wed Aug 14 2002, Hits: 390, Reviews: 0 Write review -
Purificaiton of Protein by Affinity Chromatography
(Krause Lab)
http://www.utoronto.ca/krause/affinity.html
Added: Tue May 14 2002, Hits: 787, Reviews: 0 Write review Cached -
Purification Scheme for Ubiquitin Expression Lysate
(Sosnick Lab, University of Chicago)
cation exchange column method
http://sosnick.uchicago.edu/purify_ubiquitin.html
Added: Tue Aug 13 2002, Hits: 101, Reviews: 0 Write review Cached -
Purification of MBP (maLTose-Binding Proteins) Fused Proteins
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 101, Reviews: 0 Write review Cached -
Removal of Nucleic Acids from Protein
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
different methods including polyethyleneimine (PEI) precipitation, streptomycin sulfate precipitation and polyethyleneimine (PEI) precipitation
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 1491, Reviews: 0 Write review Cached -
Small Scale MBP-fusion Protein Purification
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 232, Reviews: 0 Write review Cached -
Subcellular Fractionation of Proteins
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 430, Reviews: 0 Write review Cached -
Sucrose Density Gradient Fractionation of Yeast Membranes
(Dohlman Lab)
This is an excellent protocol for localization of membrane associated proteins in yeast.
http://www.med.unc.edu/~hdohlman/sucrose.html
Added: Tue May 14 2002, Hits: 581, Reviews: 0 Write review Cached -
Test Tube for Ion Exchange Chromatography
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
The purpose of the test tube is to find proper binding conditions such as pH and resin type, of the target protein (or contaminating proteins) to IEX (ionic exchange) resins. Once you know the best pH binding conditions, a second test tube at different salt concentrations will provide information about washing and elution conditions.
http://wolfson.huji.ac.il/purification/Protocols/T...
Added: Sat Feb 21 2009, Hits: 305, Reviews: 0 Write review Cached