Protocols
- Home-Made Taq Polymerase Purification (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Taq polymerase can be expensive. Here is the protocol for making your own Tag polymerase and it gives stable results.
http://www.nottingham.ac.uk/~mbzspd/methods/Home_m...
Added: Sat Apr 10 2004, Hits: 435, Reviews: 0 Write review Cached -
Actin Purification
(iProtocol)
Actin is the most abundant muscle and non-muscle cytoskeletal protein.
http://iprotocol.mit.edu/protocol/299.htm
Added: Fri Oct 17 2003, Hits: 205, Reviews: 0 Write review Cached -
CD2 Protein Purification
(Chen's Lab)
http://www.biochem.ucl.ac.uk/~chen/protocols/cd2pu...
Added: Tue May 14 2002, Hits: 978, Reviews: 0 Write review -
Preparation of Brain Cytosol
(Upstate)
Brain cytosolic protein preparation for general use.
http://www.upstate.com/misc/protocols.q.prot.e.bra...
Added: Tue Feb 24 2004, Hits: 705, Reviews: 0 Write review Cached -
Protein purification: tropomyosin
(iProtocol)
Method for purifying tropomyosin, an ubiquitous actin binding protein arranged in alpha-helical coiled-coil of 410 Angstrom length.
http://iprotocol.mit.edu/protocol/307.htm
Added: Fri Oct 17 2003, Hits: 117, Reviews: 0 Write review Cached -
Purification of ICAM-1
(Springer Lab, Harvard University)
The purification of ICAM-1 on R6.5 anti-ICAM-1 column.
http://www.cbrinstitute.org/labs/springer/protocol...
Added: Wed Aug 14 2002, Hits: 357, Reviews: 0 Write review Cached -
Purification of TFIIIC from Yeast
(Hahn Lab, Fred Hutchinson Cancer Research Center)
TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope. The flag purified TFIIIC is then further purified by anion exchange on a Source Q column.
http://labs.fhcrc.org/hahn/Methods/biochem_meth/tf...
Added: Thu Mar 26 2009, Hits: 647, Reviews: 0 Write review -
Skeletal Muscle Myosin Purification
(iProtocol)
A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion consists of an alpha-helical coiled-coil rod of 150 nm in length.
http://iprotocol.mit.edu/protocol/306.htm
Added: Fri Oct 17 2003, Hits: 121, Reviews: 0 Write review Cached -
Troponin Protein Purification
(iProtocol)
Troponins are calcium-dependent regulatory protein complex located on the thin actin filaments of muscle comprises of TnC (17.8 kDa), TnI (20.8 kDa), and TnT (30.5 kDa). These proteins are involved in the key regulatory mechanism for muscle contration.
http://iprotocol.mit.edu/protocol/308.htm
Added: Fri Oct 17 2003, Hits: 99, Reviews: 0 Write review Cached