Related Categories:
Protocols
- Freeze-Thaw Lysis for Mammlian Cells
(Tansey Lab at Cold Spring Harbor Laboratory)
This extraction procedure is a great way to make very concentrated extracts for Western blotting, and is particularly useful when studying low-abundance proteins. The protocol is described for harvesting mammalian cells from 6 or 10 cm tissue-culture dishes.
http://tanseylab.cshl.edu/pdf/Freeze_Thaw_Lysis.pd...
Added: Sat Mar 18 2006, Hits: 8357, Reviews: 0 Write review -
Bacterial Protein Extraction
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
mini-scale sonication method
http://www.ls.huji.ac.il/~purification/TagProteinP...
Added: Sat Jun 19 2004, Hits: 5112, Reviews: 0 Write review Cached -
Bacterial Protein Extraction Using B-Per
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
A useful procedure for initial screening of expression conditions instead of sonication of bacteria; not recommended for large scale preparation of protein
http://www.ls.huji.ac.il/~purification/TagProteinP...
Added: Sat Jun 19 2004, Hits: 1821, Reviews: 0 Write review Cached -
Preparation of Cell Lysate
(UpState)
General procedures for preparing cell lysate from adherent cells and non adherent cells for protein analysis.
http://www.millipore.com/techpublications/tech1/mc...
Added: Tue Feb 17 2004, Hits: 18322, Reviews: 0 Write review Cached -
Preparation of cell lysates from E. coli by enzymatic lysis
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 4588, Reviews: 0 Write review Cached -
Preparation of cell lysates from E. coli by sonication
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 6025, Reviews: 0 Write review Cached -
Preparation of cell lysates from E. coli using a French Press
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 2435, Reviews: 0 Write review Cached -
Preparation of cell lysates from yeast by enzymatic digestion of the cell wall
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 1664, Reviews: 0 Write review Cached -
Preparation of cell lysates from yeast using a French Press
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 1327, Reviews: 0 Write review Cached -
Preparation of cell lysates from yeast using glass beads vortexing
(The Protein Expression and Purification Facility, EMBL)
http://www.embl.de/ExternalInfo/protein_unit/draft...
Added: Sat Sep 21 2002, Hits: 1754, Reviews: 0 Write review Cached
Trouble Shootings and FAQs
- Cell lysis- How do I decide what solution to use?
- Tips on lysing CHO and HEK Mammalian Cells for SDS-PAGE
- Tips on sonication of PBMC
- Problem with protein lysates preparation
- Methods for mechanical disruption of cell
- Cell lysis for peptide binding assay
- Can I freeze down cell lysate?
- Concentrate lysate by TCA precipitation