Protocols
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"Quick and Dirty" Nuclear Extracts
(Morrisey Lab, University of Pennsylvania)
Simple method for crude nuclear extracts
http://www.uphs.upenn.edu/mcrc/morrisey/nucextract
Added: Mon Oct 14 2002, Hits: 14920, Reviews: 0 Write review Cached -
Extract Preparation
(Brent Graveley)
Preparation of nuclear extract and cytoplasmic extract
http://penguin.uchc.edu/~intron/Protocols/NuclearS...
Added: Tue May 14 2002, Hits: 8791, Reviews: 0 Write review Cached -
HeLa Cell Nuclei Preparation
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
Prepare nuclear extract from HeLa cell
http://qcom.etsu.edu/biochem/protocols/hela.htm
Added: Tue May 14 2002, Hits: 1086, Reviews: 0 Write review Cached -
Nuclear Extract Preparation
(The Morimoto Lab, Northwestern University)
Preparing nuclear extracts for in vitro transcription, footprinting, etc.
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 29 2004, Hits: 19905, Reviews: 0 Write review -
Pol II yeast nuclear extract prep (small scale)
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/biochem_meth/po...
Added: Tue May 14 2002, Hits: 1718, Reviews: 0 Write review Cached -
Preparation of HeLa Nuclear Extract
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
http://qcom.etsu.edu/biochem/protocols/hela.htm
Added: Tue May 14 2002, Hits: 1085, Reviews: 0 Write review Cached -
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots
(Mirmira Laboratory at the University of Virginia)
Step-by-step procedure and receipes.
http://faculty.virginia.edu/mirmira/resources_file...
Added: Sat Jun 05 2004, Hits: 11248, Reviews: 0 Write review Cached -
Yeast Nuclei Isolation
(Hahn Lab, Fred Hutchinson Cancer Research Center)
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when the nuclei are salt extracted and the resulting proteins AMSO4 precipitated. The high spermidine and detergent in nuclei isolation buffer are important for isolation of intact nuclei free of cellular debris.
http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
Added: Thu Mar 26 2009, Hits: 2281, Reviews: 0 Write review Cached