Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

protease inhibition - how to inhibit proteases? (Feb/26/2002 )

Hi All
Im trying to purify recombinant protein from Coli cells (invitrogen one-shot cells). But during lysis of the cells my protein seem to be degraded. How can I prevent this?

-Silas Bruun-

there are comercially available protease inhibitors, such as benzamidine or PMSF.  

There are other protease inhibitor mixes you can purchase also.

that should decrease amount of degradation you get.