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serine protease - (Mar/15/2007 )

hi all,
i am new to this forum.
I am trying to express an insect gut trypsin in pichia pastoris, my construct has his and myc tags in pPICZalpha vector, however, the protein is not being secreted to the medium, i could see some immunoreactivity wehn i tried with cell lysate protein preperation. has anybody any idea, why it might not be secreted into the medium?

-prashant pyati-

Does your plasmid direct proteins to the medium? Or do you have an export signal?