Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

Protease cutting at KK (lysine-lysine) points - know of any..?? (Feb/17/2007 )

My protein shows degradation products which show molecular weights similar to, if they were cut at lysine-lysine. Does anyone know of any proteases or something that cuts at such a position specifically. My protein has 3 such sites and all possible degradation products are seen on my SDS_PAGE.

Any ideaaaaas??!!!

Thankssssssssssssssssssss!! wink.gif


Trypsin is supposed to cut the residue after lysine and arginine (positively charged) residues. I think that would give your degradation pattern.