181. Problems with transgene expression using pcDNA3 - (reply: 5)
183. What are the main criteria for best transfection/infection? - (reply: 3)
185. reporter vector cotransfection - (reply: 4)
187. Stable transfection using plasmid with no selection gene - (reply: 2)
189. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
191. Transient transfection of MEFs - (reply: 3)
193. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
195. problems with transient transfections - (reply: 2)
197. Loss of GFP - (reply: 2)
199. viral transduction AND lipofection of neurons - (reply: 1)
201. DNA size related to the transfection efficiency？ - (reply: 9)
203. Scooping 293H colonies - (reply: 3)
205. Lenti/Retro:What happens if I.. - (reply: 2)
207. can cells just stop having the gene? - (reply: 4)
209. HELP with stable transfection - (reply: 3)

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