# transfection in neuron - (Apr/18/2007 )

I transfected a GFP tag vector in culture neuron, while it showed that no neuron was transfected, but glial cells showed much higher transfection efficiency.

Does any one know how to solve this problem?

Thanks!

-Zhiyi-

QUOTE (Zhiyi @ Apr 18 2007, 10:51 PM)

I transfected a GFP tag vector in culture neuron, while it showed that no neuron was transfected, but glial cells showed much higher transfection efficiency.

Does any one know how to solve this problem?

Thanks!

Does any one know how to solve this problem?

Thanks!

up,

up!

-Zhiyi-

what system did you use for your transfection experiment?

-genehunter-1-

QUOTE (Zhiyi @ Apr 18 2007, 08:51 AM)

I transfected a GFP tag vector in culture neuron, while it showed that no neuron was transfected, but glial cells showed much higher transfection efficiency.

Does any one know how to solve this problem?

Thanks!

Does any one know how to solve this problem?

Thanks!

Some promoters donot work in neurons but will work in glial cells. I am guessing this is the problem. Chnage promoter to a neuron specific one and the neurons will light up as well.

-scolix-

QUOTE (Zhiyi @ Apr 18 2007, 06:51 AM)

Does any one know how to solve this problem?

Thanks!

I Zhiyi,

I have transfected a plasmid coding GFP onto Primary Rat Hippocampal Neuron and I had similar problems at the beginning. I advice you to use DreamFect Gold + Combimag as transfection reagent. CombiMag are magnetic beads employed with the transfection reagent and are suitable for enhancing the efficiency of the transfection reagent. I obtained good results.

Hope that helps

-loli13-