211. HELP with stable transfection - (reply: 3)
213. G418 Killing curve - How to determinate the right G418 concentration by killing curve ? (reply: 5)
215. bad cell condition for transfection - (reply: 2)
217. HEKfectin - (reply: 3)
219. fungal contamination - fungal in cell culture (reply: 4)
221. When double-transfecting, do both plasmids always go together? - (reply: 4)
223. How many plasmid DNA copies per cell? - (reply: 1)
225. G418 resistant 293 - (reply: 4)
227. loss of GFP upon selection - (reply: 4)
229. What is the right G418 concentration for selection on TSA and HEK? - (reply: 8)
231. Is my geneticin bad or do I use it in a wrong way? - (reply: 7)
233. CAn trypsin be used for cell harvest? - (reply: 4)
235. When are the cells virus free? - (reply: 6)
237. what transfection effeciency is needed for westen? - (reply: 2)
239. Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)

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