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271. have you ever used hygromycin to screen transfected HepG2 or gastric cancer cell - all the cells died, why? (reply: 1)
273. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
275. HEK293 WT showing resistance to G418 - Please, I need ideas and opinions (reply: 8)
277. Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
279. stable cell line questions( cell type matter) - (reply: 2)
281. Normal Human Fibroblasts - (reply: 1)
283. selecting single cell colonies using single cell per well dilution one day after - (reply: 1)
285. Infection efficiency of non-adherent cells - (reply: 3)
287. electroporation - why glycerol? (reply: 2)
289. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
291. Phenol/Chlorophorm extracted DNA - ok to use for transfection??? (reply: 2)
293. Trouble transfecting particular construct - (reply: 1)
295. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
297. Hela cells and stable transfection help please! - (reply: 6)
299. antibiotic selection for breast cancer MDA-MB-436 - (reply: 1)
273. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
275. HEK293 WT showing resistance to G418 - Please, I need ideas and opinions (reply: 8)
277. Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
279. stable cell line questions( cell type matter) - (reply: 2)
281. Normal Human Fibroblasts - (reply: 1)
283. selecting single cell colonies using single cell per well dilution one day after - (reply: 1)
285. Infection efficiency of non-adherent cells - (reply: 3)
287. electroporation - why glycerol? (reply: 2)
289. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
291. Phenol/Chlorophorm extracted DNA - ok to use for transfection??? (reply: 2)
293. Trouble transfecting particular construct - (reply: 1)
295. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
297. Hela cells and stable transfection help please! - (reply: 6)
299. antibiotic selection for breast cancer MDA-MB-436 - (reply: 1)