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31. Coating Protein A and G on ELISA plate - (reply: 2)
32. coating Serum - (reply: 2)
33. Coating with monoclonal/polyclonal - (reply: 6)
34. sandwich elisa - how to increase O.D. (reply: 4)
35. how do u determine actual concentration from absorbance readings? - using tissue lysates for ELISA sample (reply: 3)
36. ELISA protocol (indirect and sandwich) and troubleshooting tips - from abcam (reply: 3)
37. Steroid coating - I'm looking a protocol for steroid ELISA. (reply: 4)
38. Calibration of internal standards - (reply: 1)
39. Can we re-use antibody solutions just like Western Blot? - to save antibody (reply: 3)
40. choosing the best ELISA method - used to do Immunoprecipitation (reply: 2)
41. Serial dilutions - (reply: 1)
42. ELISA result interpretation - (reply: 2)
43. eNOS ELISA - (reply: 2)
44. ELISA Results - (reply: 1)
45. Blocking buffer recipe - (reply: 5)
46. reaction buffer for ABTS - any protocol? (reply: 1)
47. High sample readings in serum ELISA - Please help... (reply: 2)
48. Culture media with human serum gives false positive - (reply: 1)
49. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
50. alternative coating buffers for ELISA - anything else i can use aside from PBS and carbonate/bicarbonate? (reply: 7)
51. ELISA- colormetric? chemilluminescence? spectrophotometic? - (reply: 4)
52. hGH ELISA - (reply: 1)
53. Using H2O to wash ELISA plate? - (reply: 8)
54. Exposure time when measuring cytokines? - (reply: 2)
55. MIP-2 ELISA detailed questions - (reply: 3)
56. ELISA Problem since change of antibody supplier? - (reply: 1)
57. antibody gold labeling - (reply: 3)
58. Elisa for total human IgG from culture supernatant - (reply: 1)
59. elisa background - (reply: 3)
60. ELISA vs Western - The two aren't agreeing.. (reply: 1)

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