Exposure time when measuring cytokines? - (Apr/29/2008 )

I noticed there are many experts in this forum on ELISA and also probably on cytokine research. Could any of you shed some light on a quite general but important issue:
Is there a best time point to choose for detecting common cytokines (TNF-alpha, IL-6, IL-8, etc), no matter what exposure it is?
"Best time" means a time at which cytokine release attains it's peak and after which the level would go down.
It may depend on type of exposure. If it's the case, I would like to know the profiles of LPS, H2O2, hyperoxide or other exposures that are frequently studied.
It could also depend on dose and cell type used in vitro or target tissue in vivo, about which I don't know.
Thanks a lot!
Hanhan

hi,

Despite all the variables, i would go for atleast 24 hours n optimal 48 hours.
u can collect good amount of variety of cytokines in the supernatant.

gud luck

Thank you! 48 hr for in vivo study, right? Could we really do 48 exposure for cultured cells?