Calibration of internal standards - (Jul/29/2008 )

If I am sent a reference serum and need to use it to calibrate my internal standards, what is the best way to accomplish that? Any help would be appreciated.

The 'serum' you are sent is the 'reference preparation'. Construct a dose response curve with this material in parallel with your in-house standards. The points you make using the reference preparation should cover the range of your standards and be higher and also lower. (So you only have to do this experiment once).

Read the results of your in-house standards as unknowns from the dose response curve constructed from the reference preparation.

Assign these values to your standards.

Your standards are now calibrated to the known reference.

You should freeze aliquotes of your standards and use these to confirm concentrations of new lots . (I assume the 'reference serum' is hard to obtain).