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New Forum Archives (2009-): :
211. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
213. Using pBABE plasmids for regular transfection - (reply: 1)
215. Cloning two genes in the same plasmid with one promotor - (reply: 4)
217. Is this counter-productive cloning or not? - (reply: 8)
219. Failed cloning for 1month - TOPO - (reply: 7)
221. Which method for DNA purification b4 cloning - (reply: 4)
223. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
225. TOPO cloning after Phusion-PCR - (reply: 1)
227. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
229. Electrophoresis samples and volumes - (reply: 1)
231. Checking for efficieny competent cells - (reply: 1)
233. Does this look alright? - (reply: 3)
235. Cloning pDsRed-Monomer-N1 - (reply: 1)
237. Ligation left for weekend at room temp - (reply: 4)
239. Problem in plasmid digestion after transformation - (reply: 4)
213. Using pBABE plasmids for regular transfection - (reply: 1)
215. Cloning two genes in the same plasmid with one promotor - (reply: 4)
217. Is this counter-productive cloning or not? - (reply: 8)
219. Failed cloning for 1month - TOPO - (reply: 7)
221. Which method for DNA purification b4 cloning - (reply: 4)
223. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
225. TOPO cloning after Phusion-PCR - (reply: 1)
227. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
229. Electrophoresis samples and volumes - (reply: 1)
231. Checking for efficieny competent cells - (reply: 1)
233. Does this look alright? - (reply: 3)
235. Cloning pDsRed-Monomer-N1 - (reply: 1)
237. Ligation left for weekend at room temp - (reply: 4)
239. Problem in plasmid digestion after transformation - (reply: 4)