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New Forum Archives (2009-): :
1471. insert is missing after TA cloning - TA cloning (reply: 6)
1473. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1475. cloning problem - molecular cloning (reply: 1)
1477. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1479. choosing restriction sites - (reply: 5)
1481. Transduction - (reply: 3)
1483. my colonies don't have the insert, how is this possible? - (reply: 7)
1485. PCR problem from transformed TOPO TA vector - (reply: 3)
1487. why sometimes big or small colonies? - (reply: 16)
1489. wrong bands after T-vector cloning - (reply: 3)
1491. two step digestion - (reply: 6)
1493. Self-Ligation of digested vector - (reply: 2)
1495. Ligation issue: Does vector size matter? - (reply: 10)
1497. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1499. transformation problem - (reply: 3)
1473. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1475. cloning problem - molecular cloning (reply: 1)
1477. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1479. choosing restriction sites - (reply: 5)
1481. Transduction - (reply: 3)
1483. my colonies don't have the insert, how is this possible? - (reply: 7)
1485. PCR problem from transformed TOPO TA vector - (reply: 3)
1487. why sometimes big or small colonies? - (reply: 16)
1489. wrong bands after T-vector cloning - (reply: 3)
1491. two step digestion - (reply: 6)
1493. Self-Ligation of digested vector - (reply: 2)
1495. Ligation issue: Does vector size matter? - (reply: 10)
1497. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1499. transformation problem - (reply: 3)