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New Forum Archives (2009-): :
1441. Odd point mutations - (reply: 23)
1443. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1445. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1447. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1449. Ligation trouble continues - subcloning ligation failure (reply: 9)
1451. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1453. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1455. Truncated sequence - (reply: 1)
1457. Purifying and cloning 69nt product - (reply: 5)
1459. ligatin issues - (reply: 1)
1461. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1463. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1465. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1467. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1469. vivo expression technology - ivet (reply: 25)
1443. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1445. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1447. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1449. Ligation trouble continues - subcloning ligation failure (reply: 9)
1451. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1453. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1455. Truncated sequence - (reply: 1)
1457. Purifying and cloning 69nt product - (reply: 5)
1459. ligatin issues - (reply: 1)
1461. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1463. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1465. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1467. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1469. vivo expression technology - ivet (reply: 25)