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New Forum Archives (2009-): :
1321. need help cloning cdna of a known protein - (reply: 1)
1323. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1325. Trouble with subcloning - (reply: 6)
1327. Lb-kan plates life - How long can they be used? (reply: 2)
1329. TOPO-Transformation Problem - (reply: 4)
1331. designing primers for genes not sequenced yet - (reply: 2)
1333. Cloning Problem - (reply: 3)
1335. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1337. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1339. Restriction buffer composition - (reply: 2)
1341. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1343. Yet another ligation problem - (reply: 4)
1345. Promoter Cloning for Luciferase Assay - (reply: 3)
1347. cloning protocol - (reply: 1)
1349. Miniprep - (reply: 3)
1323. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1325. Trouble with subcloning - (reply: 6)
1327. Lb-kan plates life - How long can they be used? (reply: 2)
1329. TOPO-Transformation Problem - (reply: 4)
1331. designing primers for genes not sequenced yet - (reply: 2)
1333. Cloning Problem - (reply: 3)
1335. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1337. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1339. Restriction buffer composition - (reply: 2)
1341. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1343. Yet another ligation problem - (reply: 4)
1345. Promoter Cloning for Luciferase Assay - (reply: 3)
1347. cloning protocol - (reply: 1)
1349. Miniprep - (reply: 3)